Pepper vein yellows virus 9: a novel polerovirus isolated from chili pepper in Indonesia
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Pepper vein yellows virus 9: a novel polerovirus isolated from chili pepper in Indonesia Sota Koeda1,2 · Kanami Homma2 · Mari Kamitani3 · Atsushi J. Nagano3 · Marina Taniguchi1 · Nadya Pohan4 · Elly Kesumawati4 Received: 8 July 2020 / Accepted: 6 September 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract In 2017, a leaf sample from a single chili pepper (Capsicum annuum) plant exhibiting yellowing was collected from Aceh province, Indonesia. Total RNA was extracted from this sample, and RNA-Seq analysis was conducted. Putative infecting viruses were detected by mapping the obtained reads to the full-length viral genome sequences available in the GenBank database (7457 sequences) and the de novo-assembled contigs. RNA-Seq analysis detected polerovirus, begomovirus, and amalgavirus sequences, and the polerovirus-like sequences showed strong similarity to those of previously reported pepper vein yellows viruses (PeVYVs). The complete viral genome sequence obtained by RT-PCR had a length of 6023 nt, had the typical genome organization of a polerovirus and showed a high degree of sequence similarity to PeVYV-2 from Israel. Moreover, the predicted amino acid sequence of the P0 protein of the Indonesian isolate was 85.1% to 88.8% identical to those of other PeVYVs. In accordance with the polerovirus species demarcation criteria, this isolate should be assigned to a new polerovirus species, and we propose the name “pepper vein yellows virus 9” (PeVYV-9) for this virus. The family Luteoviridae is composed of members of the genera Enamovirus, Luteovirus, and Polerovirus and seven other unassigned viruses (ICTV). The polerovirus genome is a linear, non-segmented, positive-sense ssRNA with an approximate length of 5.6–6.2 kb [1]. Poleroviruses are generally transmitted by aphids, but some have been reported to be transmitted by whiteflies (Bemisia tabaci) [2–4]. Pepper vein yellows virus (PeVYV), which induces symptoms of vein yellowing and leaf roll in pepper (Capsicum spp.) plants Handling Editor: Elvira Fiallo-Olivé. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04838-6) contains supplementary material, which is available to authorized users. * Sota Koeda [email protected] 1
Faculty of Agriculture, Kindai University, Nara, Nara 631‑8505, Japan
2
Graduate School of Agriculture, Kindai University, Nara, Nara 631‑8505, Japan
3
Faculty of Agriculture, Ryukoku University, Otsu, Shiga 520‑2194, Japan
4
Faculty of Agriculture, Syiah Kuala University, Darussalam, Banda Aceh 23111, Indonesia
were initially reported and identified in Japan (PeVYV-1) [5, 6]. After that, members of seven additional species (PeVYV-2 to 8) were reported in Israel, China, Australia, Spain, Greece, Saudi Arabia, and Brazil, and PeVYV is becoming a serious global threat to pepper production [1, 7–12]. Fresh chili pepper is an essential spice in Indonesian cuisine, and according to statistics from the Food and Agricu
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