Performance of concanavalin A-immobilized on polyacrylate beads for the detection of human norovirus and hepatitis A vir
- PDF / 235,511 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 21 Downloads / 157 Views
Performance of concanavalin A-immobilized on polyacrylate beads for the detection of human norovirus and hepatitis A virus in fecal specimens Songhak Kim1 • Susanne U. Mertens-Talcott2 • Bipin Vaidya1 • Vinicius Paula Venancio2 • Se-Young Cho1 • Jong-Am Song3 • Boon P. Chew2 • Joseph Kwon3 • Duwoon Kim1
Received: 15 June 2020 / Revised: 15 September 2020 / Accepted: 25 September 2020 Ó The Korean Society of Food Science and Technology 2020
Abstract Quantitative reverse transcription PCR (qRTPCR) is a sensitive method for the detection of foodborne viruses in fecal samples. However, the performance of qRT-PCR depends on the efficiency of virus concentration methods. In this study, the effect of Concanavalin A (Con A)-immobilized on polyacrylate beads (Con A-PAB) on the qRT-PCR performance, in terms of sensitivity and specificity to detect foodborne viruses in human fecal specimens was compared with commercial viral RNA extraction kit (VRNA). The detection of foodborne viruses by qRT-PCR was validated by viral genome sequencing. Both Con A-PAB and VRNA methods were equally sensitive and specific for detecting hepatitis A virus in fecal specimens. Even though both methods showed high specificity (100% vs. 100%) for detecting human norovirus (HuNoV), Con A-PAB method exhibited higher sensitivity (100% vs. 42.9%) and accuracy (100% vs. 73.3%) compared to VRNA method. In conclusion, the application of Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10068-020-00833-4) contains supplementary material, which is available to authorized users.
Con A-PAB would improve the performance of qRT-PCR for the detection of HuNoV in fecal samples. Keywords Concanavalin A Hepatitis A virus Human norovirus Sensitivity Specificity
Introduction Foodborne pathogens are recognized as a significant food safety hazard and represent the most reported cause of illness outbreaks worldwide (WHO, 2015). Human norovirus (HuNoV) and hepatitis A virus (HAV) are the most concerned pathogens due to high incidence of morbidity and mortality, and potential for transmission via contaminated food (FAO/WHO, 2012). HuNoV and HAV are responsible for viral gastroenteritis and viral hepatitis outbreaks worldwide, respectively. The human gastrointestinal tract is a reservoir of these viruses, (Randazzo et al., 2018) and human feces remains a potential source of Jong-Am Song [email protected]
& Duwoon Kim [email protected]
Boon P. Chew [email protected]
Songhak Kim [email protected]
Joseph Kwon [email protected]
Susanne U. Mertens-Talcott [email protected] 1
Vinicius Paula Venancio [email protected]
Department of Food Science and Technology and Foodborne Virus Research Center, Chonnam National University, Gwangju 61186, Republic of Korea
2
Se-Young Cho [email protected]
Department of Nutrition and Food Science, Texas A&M University, College Station, TX 77843-2252, USA
3
Biological Disaster Analysis Group, Korea Basic Science Institute, Daejeon 34133, Republic of Korea
Bipin Vaidya bi
Data Loading...
