Periodontal Injection of Lipopolysaccharide Promotes Arthritis Development in Mice
- PDF / 2,179,878 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 63 Downloads / 208 Views
ORIGINAL ARTICLE
Periodontal Injection of Lipopolysaccharide Promotes Arthritis Development in Mice Anna Scanu,1,8 Chiara Giraudo,2 Francesca Galuppini,3,4 Vanni Lazzarin,3 Gianmaria Pennelli,3 Stefano Sivolella,5 Edoardo Stellini,5 Francesca Oliviero,1 Paola Galozzi,1 Massimo Rugge,3 Roberto Stramare,2 Roberto Luisetto,6 and Leonardo Punzi7
Abstract— This study evaluated the arthritogenic effect of lipopolysaccharide (LPS) in a mouse
model of periodontal disease. Periodontitis was induced in wild-type CD1 mice by nine LPS injections (10 or 50 ng) into the maxillary mucosa. Untreated mice or injected with LPS at the tail were used as controls. Two weeks after final inoculation, mice were sacrificed to collect blood, maxilla, and paw samples. Development and progression of periodontitis and arthritis were monitored using clinical assessment, micro-computed tomography (micro-CT), ultrasound (US), and histological analysis. CXCL1, IL-1β, IL-6, TNF-α, and anti-citrullinated peptide antibodies (ACPA) serum levels were determined by enzyme immunoassay. Ankle swelling and inflammation manifested after the 5th periodontal injection of 50 ng of LPS and progressed until the end of experiments. Periodontal injection of 10 ng of LPS and LPS tail injection did not induce paw changes. Therefore, the subsequent assessments were conducted only in mice periodontally injected with 50 ng of LPS. Maxillary micro-CTand histological analysis showed that LPS-induced alveolar bone resorption and vascular proliferation in periodontal tissue, but not inflammation. US and histology revealed increased joint space, leukocyte infiltration, synovial proliferation, and mild cartilage and bone destruction in the paws of mice orally injected. Cytokines and ACPA showed a trend towards an increase in LPS mice. This study shows that arthritis and periodontal disease can co-occur in wild-type mice after periodontal injection of LPS at optimal dose. Our model may be useful to improve the understanding of the mechanisms linking periodontitis and arthritis. KEY WORDS: periodontitis; arthritis; inflammation; mouse model; periodontal disease; animal model. Rheumatology Unit, Department of Medicine – DIMED, University of Padova, Via Giustiniani, 2, 35128 Padova, Italy 2 Radiology Unit, Department of Medicine – DIMED, University of Padova, Padova, Italy 3 Surgical Pathology Unit, Department of Medicine – DIMED, University of Padova, Padova, Italy 4 Department of Women’s and Children’s Health, Padova, Italy 5 Dentistry Section, Department of Neurosciences, University of Padova, Padova, Italy 6 Department of Surgical Oncological and Gastroenterological Sciences, University of Padova, Padova, Italy 7 Centre for Gout and Metabolic Bone and Joint Diseases, Rheumatology, SS Giovanni and Paolo Hospital, Venice, Italy 8 To whom correspondence should be addressed at Rheumatology Unit, Department of Medicine – DIMED, University of Padova, Via Giustiniani, 2, 35128 Padova, Italy. E-mail: [email protected] 1
INTRODUCTION Periodontitis is a very common oral chronic
Data Loading...
