Phase Inversion Molecularly Imprinting of Uracil Targeted Membranes Made of Polyacrylonitrile Copolymers Having Methacry
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Phase Inversion Molecularly Imprinting of Uracil Targeted Membranes Made of Polyacrylonitrile Copolymers Having Methacrylic Acid and Acrylic Acid Segments for Recognition and Permselective Binding Shao Ling Xia1, Hong Ying Wang1, Takaomi Kobayashi Department of Chemistry, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, JAPAN 940-2188 1 Material School of Zhengzhou University, Zhengzhou 450052, P. R. China ABSTRACT Polyacrylonitrile having methacrylic acid [P(AN-co-MAA)] and acrylic acid [P(AN-co-AA)] was used for phase inversion imprinting of uracil (URA). Resultant imprinted membranes had porous morphology and showed permselective binding of URA, when 32 µM URA aqueous solution was permeated under pressure gradient of 100 Pa across the membrane. Under the circumstance, the imprinted P(AN-co-MAA) membrane bound 7.9 µmol/g of URA with 8.4x10-6 m3/m2·s of volume flux. The imprinted P(AN-co-AA) membrane showed loose selectivity relative to the P(ANco-MAA) membrane. Binding behavior of URA and dimethyluracil (DMURA) was also compared in both imprinted membranes. Evidence was presented that P(AN-coMAA) with methacrylic segments was effective for URA imprinting and resulted in high URA selectivity in the permselective binding experiments. INTRODUCTION Molecular imprinting is an effective method to create selective recognition sites in synthetic polymers [1]. As tailor made polymer, molecular imprinted polymers (MIPs) have been applied in several fields [2, 3]. In recent works of MIPs, a challenge has paid to membrane, since imprinted membranes have been attracted much interest in view of research subjects to separation fields [4-7]. But little is known about imprinted membrane with recognition and separation in permselective binding to target molecule. Recently, we have developed phase inversion imprinting technique, which used scaffolding polymers with functional groups for theophylline (THO) [8-10], amino acids [11] and dibenzofuran [12]. In the technique, coagulation process of polymer is applied to imprint information of template molecules. Resultant polymeric membranes showed permselective binding to target molecule. In the present study, we have imprinted URA by using phase inversion imprinting of acrylonitrile copolymers with methacrylic acid (MAA) and acrylic acid (AA) (Scheme). Since application of the URA imprinting is meaningful in preparation of
G7.5.1
G7.5.2
synthetic receptor, it is also useful to develop novel bio-mimetic artificial materials. We have focused on phase inversion imprinting of P(AN-co-MAA) and P(AN-coAA) for URA recognition. Here, P(AN-co-MAA) consists of MAA with methyl group, while P(AN-co-AA) has AA without methyl group. Effect of MAA and AA in the imprinted membrane is emphasized on the permselective binding of URA. (CH2-CH)
x
CN
CH3 (CH 2-C) y (CH2-CH) x C=O CN OH
P (AN-co-MAA)
(CH 2-CH) y C=O OH
H N
O H
P(AN-co-AA)
N
O URA
CH3 N
O H3C
N
O DMURA
Scheme Chemical structures of P(AN-co-MAA), P(AN-co-AA), URA template and DMURA. EXPERIMENTAL DETAILS P(AN-co-
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