Physical Activity During Pregnancy Is Associated with Increased Placental FATP4 Protein Expression
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ORIGINAL ARTICLE
Physical Activity During Pregnancy Is Associated with Increased Placental FATP4 Protein Expression Kelly Ann Hutchinson 1 & Nhung H. Vuong 1 & Shuhiba Mohammad 1 & Catherine Everest 1 & Macyn L. Leung 1 & Jayonta Bhattacharjee 1 & Kristi B. Adamo 1 Received: 16 January 2020 / Accepted: 6 May 2020 # Society for Reproductive Investigation 2020
Abstract Placental function is of utmost importance to ensure proper fetal development in utero. Among the placenta’s many roles includes the passage of sufficient macronutrients, such as glucose, amino acids, and fatty acids, to the fetus. Macronutrients are carried from maternal circulation to the fetus across transporters within the placenta. The objective of this study was to examine the impact of (i) an acute bout of exercise and (ii) chronic exercise participation on placenta nutrient transporter expression and localization. To investigate the effect of acute exercise, pre- and post-exercise serum was collected from pregnant (n = 5) and nonpregnant (n = 5) women who underwent a moderate-intensity exercise session and used to treat BeWo cells. To assess chronic physical activity, we analyzed term placenta from women categorized as active (n = 10) versus non-active (n = 10). Protein expression and localization for the transporters GLUT1, SNAT1, and FATP4 were examined for both groups. GLUT1 expression in BeWo cells treated with serum from pregnant women was higher compared with that from non-pregnant, independent of exercise. FATP4 protein expression was elevated in the term placenta of active women. Immunohistochemistry analysis of term placenta illustrated increased staining of FATP4 in placental tissue from active women and differential staining pattern of GLUT1 depending on physical activity status. Chronic exercise during pregnancy increases the expression of placental FATP4 in vivo, suggesting greater metabolism and usage of fatty acids. Additionally, serum from pregnant women could contain factors that increase GLUT1 protein expression in vitro. BeWo cells treated with pre- and post-exercise serum from pregnant women resulted in greater GLUT1 expression compared with those treated with pre- and post-exercise serum from non-pregnant women. Physical activity appears to differentially impact key placental transporters involved in the transfer and availability of nutrients from mother to fetus. Future research ought to examine the mechanisms involved in regulating these changes and their impact on fetal growth and health. Keywords Physical activity . Placenta . Pregnancy . Nutrient transport . Trophoblast
Introduction The fetus is reliant on the maternal supply of nutrients via the placenta. Placental growth and function are therefore critical for fetal development and long-term health outcomes. Research shows that increased or decreased nutrient transport is associated with excessive or restricted fetal growth, respectively [1, 2]. Parallel to these findings, maternal pregnancy-
* Kristi B. Adamo [email protected] 1
Present address: Faculty of Heal
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