Plant Functional Genomics Methods and Protocols
This second edition volume discusses the revolutionary development of faster and less expensive DNA sequencing technologies from the past 10 years and focuses on general technologies that can be utilized by a wide array of plant biologists to address spec
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Jose M. Alonso Anna N. Stepanova Editors
Plant Functional Genomics Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Plant Functional Genomics Methods and Protocols Second Edition Edited by
Jose M. Alonso Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, USA
Anna N. Stepanova Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, USA
Editors Jose M. Alonso Department of Plant and Microbial Biology North Carolina State University Raleigh, NC, USA
Anna N. Stepanova Department of Plant and Microbial Biology North Carolina State University Raleigh, NC, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2443-1 ISBN 978-1-4939-2444-8 (eBook) DOI 10.1007/978-1-4939-2444-8 Library of Congress Control Number: 2015933161 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Since its conception in the 1990s, the term Functional Genomics has been used to describe a very diverse and constantly evolving set of experimental and computational disciplines aimed at deciphering the function of the thousands of genes encoded in a genome. Understanding the contribution of each gene in an organism is an extraordinary challenge, not only because of the large number of genes involved but also due to the multitude of facets that comprise a “gene’s function,” from the elementary biochemical activity of the encoded product, to the mechanisms of its regulation, to the role of the gene in the context of all other
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