Platinum nanoflowers with peroxidase-like property in a dual immunoassay for dehydroepiandrosterone
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ORIGINAL PAPER
Platinum nanoflowers with peroxidase-like property in a dual immunoassay for dehydroepiandrosterone Huiyi Yang 1 & Qiyi He 1 & Yingshan Chen 1 & Ding Shen 1 & Huanxin Xiao 1 & Sergei A. Eremin 2 & Xiping Cui 1 & Suqing Zhao 1 Received: 18 March 2020 / Accepted: 21 August 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Platinum nanoflowers (PtNFs) were utilized in a competitive enzyme-linked immunosorbent assay (ELISA) and in a lateral flow immunoassay (LFIA) for superior peroxidase-like activity and intense brown color, respectively. PtNFs were linked to the polyclonal antibody (pAb) to form the pAb-PtNFs probes for the dual immunoassay. Based on optimized pAb-PtNF probes, both enzyme-linked immunosorbent assay (PtNFs-ELISA) and lateral flow immunoassay (PtNFs-LFIA) perform very well. The absorbance at 450 nm decreases linearly in the DHEA concentration range 2.1 to 118.1 ng mL−1, and the limit of detection is 1.3 ng mL−1 and the IC50 value is 15.7 ng mL−1 of PtNFs-ELISA. The visual cut-off value of PtNFs-LFIA is 10.0 ng mL−1. The average recoveries from spiked samples range from 95.0 to 108.9% with a coefficient of variation below 12.2%. Excellent recoveries and correlation between the two methods were observed. Furthermore, the designed immunosensors exhibited good selectivity, confirming a broad development prospect in DHEA monitoring. Keywords Dehydroepiandrosterone (DHEA) . Platinum nanoflowers (PtNFs) . ELISA . LFIA . Immunoassays . Polyclonal antibody
Introduction Micromolecule metabolites, the intermediates and products of metabolism, are now emerging as a significant type of biomarkers for monitoring and diagnosis of various threatening diseases [1, 2]. Dehydroepiandrosterone (DHEA) and its
sulfated metabolite (DHEAs) are highly sensitive and reliable markers of adrenocortical tumors as when tumor masses gradually increase when they are yet detectable by imaging methods [3]. Adrenocortical carcinoma (ACC) is an exceedingly rare malignancy, which is a continuing challenge considering the uncertainty or absence of proper histopathologic evaluation and
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04528-9) contains supplementary material, which is available to authorized users. * Xiping Cui [email protected]
Huanxin Xiao [email protected]
* Suqing Zhao [email protected]
Sergei A. Eremin [email protected]
Huiyi Yang [email protected] Qiyi He [email protected]
1
Department of Pharmaceutical Engineering, School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, People’s Republic of China
2
Faculty of Chemistry, M. V. Lomonosov Moscow State University, 119991 Moscow, Russia
Yingshan Chen [email protected] Ding Shen [email protected]
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the limited availability of tools for ACC treatment. Precise quantification of DHEA is essential to define the status of cancer patients [4, 5]. For healthy people, the concentration of DHEA in urin
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