Preparation and Characterization of Nano-Laponite/PLGA Composite Scaffolds for Urethra Tissue Engineering
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ORIGINAL PAPER
Preparation and Characterization of Nano‑Laponite/PLGA Composite Scaffolds for Urethra Tissue Engineering Zhen Wang1 · Jinhua Hu2 · Jiakang Yu2 · Daquan Chen1
© Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The purpose of this study was to construct a biomimetic urethral repair substitute. The nano-Laponite/polylactic acid–glycolic acid copolymer (PLGA) fiber scaffolds were produced to replicate the natural human urethra tissue microenvironment. PLGA (molar ratio 50:50) and Laponite were used in this study as raw materials. The nano-Laponite/PLGA scaffolds were fabricated via electrospinning technology. After preparing the material, the microstructural and mechanical properties of the nano-Laponite/PLGA scaffold were tested via scanning electron microscopy and electronic universal testing. The effects of different amounts of Laponite on the degradation of the nano-Laponite/PLGA scaffold were studied. Human umbilical vein endothelial cells (HUVECs) were co-cultured with PLGA and nano-Laponite/PLGA scaffolds for 24, 48, or 72 h. Scanning electron microscopy results illustrated that the microstructure of the scaffold fabricated by electrospinning was similar to that of the natural extracellular matrix. When the electrospinning liquid contained 10% Laponite, the nano-Laponite/PLGA stress–strain curve illustrated that the scaffold has strong elastic deformation ability. HUVECs exhibited good growth on the nano-Laponite/PLGA scaffold. When the scaffold contained 1% Laponite, the cell proliferation rate in the CCK-8 test was significantly better than that for the other three materials, displaying good cell culture characteristics. The 1% nanoLaponite/PLGA composite scaffold can be used as a suitable urethral repair material, but its performance requires further development and research. Keywords Laponite · PLGA · Urethral stent · Electrospinning · HUVECs
Introduction Congenital urethral malformation caused by trauma and infection often requires urethral reconstruction to prevent morbidity or mortality. To restore the function of the urethra after damage and facilitate wound healing, artificial Zhen Wang and Jinhua Hu have contributed equally to this work. * Jiakang Yu [email protected] * Daquan Chen [email protected] 1
School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University, Yantai 264005, China
Guangzhou Women and Children’s Medical Center, Guangzhou 510623, Guangdong province, China
2
substitutes are needed to construct a biomimetic urethral repair scaffold [1–3]. For patients experiencing long-term urethral stricture in particular, repair is urgently needed. At present, autologous grafts obtained from patients such as the foreskin flap and tongue mucosa have been used to mimic the structure and composition of the natural urethral tissue. Unfortunately, the avail
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