Preparation and comparison of Fe 3 O 4 @graphene oxide nanoclusters for analysis of glimepiride in urine by surface-assi

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RESEARCH PAPER

Preparation and comparison of Fe3O4@graphene oxide nanoclusters for analysis of glimepiride in urine by surface-assisted laser desorption/ionization time-of-flight mass spectrometry Han-Ju Chien 1 & Syu-Ming Lai 2 & Wei-Chen Wang 1 & Hung-Yu Lin 1 & Yu-Min Juang 3 & Ping-Shan Lai 2 & Chien-Chen Lai 1,4 Received: 20 June 2019 / Revised: 11 March 2020 / Accepted: 20 March 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020

Abstract Graphene oxide (GO) has the ability to absorb certain compounds, and it can be modified with functional groups for different purposes; for instance, iron oxide (IO) nanoparticles can be used to concentrate analyte by a magnet. Recently, many kinds of GO have been developed, such as single-layer GO (SLGO), two-to-four layers of GO (i.e., few-layer GO, FLGO2–4), and four-toeight layers of GO (i.e., multi-layer GO, MLGO4–8). However, the abilities of these layered GO coated with IO nanoparticles have not been investigated. In this study, we conducted a novel analysis of glimepiride by using layered GO-coated magnetic clusters of IO nanoparticles that were synthesized through a simple and facile emulsion-solvent evaporation method. The methodology is based on (i) enrichment of glimepiride using the layered GO-coated magnetic clusters of IO nanoparticles (IO@SLGO, IO@FLGO2–4, and IO@MLGO4–8), and (ii) rapid determination using magnetic cluster–based surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOFMS). We found that IO@MLGO4–8, the magnetic cluster with the greatest number of GO layers, had the best limit of detection (28.6 pmol/μL for glimepiride). The number of GO layers played a significant role in increasing the sensitivity of the SALDI-MS, indicating that the size of GO in the magnetic clusters contributed to the desorption/ionization efficiency. To the best of our knowledge, this is the first study to enrich glimepiride using magnetic clusters of different GO types and to show that the glimepiride in HLB purified urine adsorbed by magnetic clusters can be analyzed by SALDI-TOFMS. Keywords SALDI . Nanocluster . Iron oxide . Graphene oxide . Mass spectrometry . Glimepiride

Han-Ju Chien and Syu-Ming Lai contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00216-020-02611-x) contains supplementary material, which is available to authorized users. * Ping-Shan Lai [email protected] * Chien-Chen Lai [email protected] 1

Institute of Molecular Biology, National Chung Hsing University, Taichung 40227, Taiwan

2

Department of Chemistry and Center of Nanoscience and Nanotechnology, National Chung Hsing University, Taichung 40227, Taiwan

3

Waters Corporation, Taipei City 10491, Taiwan

4

Graduate institute of Chinese Medical Science, China Medical University, Taichung 40402, Taiwan

Abbreviations CNT MTT FLGO GO IO MLGO SLGO SALDI-TOFMS TEM

Carbon nanotubes 3-(4,5-Dimethylthiazol-2yl)-2,5diphenyltetrazolium bromide Few-layer graphene oxi

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