Production and characterization of an enzyme extract with cellulase activity produced by an indigenous strain of Fusariu
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ORIGINAL RESEARCH PAPER
Production and characterization of an enzyme extract with cellulase activity produced by an indigenous strain of Fusarium verticillioides ITV03 using sweet sorghum bagasse Marı´a Ine´s Infanzo´n-Rodrı´guez . Juan Arturo Ragazzo-Sa´nchez . Sandra del Moral . Montserrat Caldero´n-Santoyo . Marı´a Guadalupe Aguilar-Uscanga
Received: 22 January 2020 / Accepted: 9 June 2020 Ó Springer Nature B.V. 2020
Abstract Objectives To evaluate a strain of Fusarium verticillioides ITV03 isolated from wood residues in the Veracruz region of Mexico. Endoglucanase and bglucosidase production by submerged fermentation was optimized using a Box–Behnken design, where the independent variables were urea, ammonium sulfate and yeast extract. Results After optimization, an endoglucanase activity of 0.27 U/mL was achieved; subsequently, three carbon sources were evaluated (carboxymethyl cellulose, sweet sorghum bagasse cellulose and delignified sweet sorghum bagasse (DSSB). The results showed M. I. Infanzo´n-Rodrı´guez J. A. Ragazzo-Sa´nchez M. Caldero´n-Santoyo Laboratorio Integral de Investigacio´n en Alimentos, Tecnolo´gico Nacional de Me´xico/Instituto Tecnolo´gico de Tepic, C.P. 63175 Tepic, NAY, Mexico e-mail: [email protected] J. A. Ragazzo-Sa´nchez e-mail: [email protected] M. Caldero´n-Santoyo e-mail: [email protected] S. del Moral M. G. Aguilar-Uscanga (&) Ca´tedra-CONACYT, Tecnolo´gico Nacional de Me´xico/ Instituto Tecnolo´gico de Veracruz-UNIDA, Av. M. A. de Quevedo Nu´m. 2779, C.P. 91860 Veracruz, VER, Mexico e-mail: [email protected] S. del Moral e-mail: [email protected]
that DSSB yielded the greatest endoglucanase (0.28 U/mL) and b-glucosidase (0.12 U/mL) activities. Both enzymatic activities were characterized for the effect of pH, temperature and thermostability. The optimal parameters of b-glucosidase and endoglucanase activity were pH 5 and 4 respectively, the optimum temperature 60 °C. These enzymes were stable at 50 °C for 150.68 h and 8.54 h, with an activation energy (Ea(day)) of 265.55 kJ/mol and 44.40 kJ/mol respectively, for b-glucosidase and endoglucanase. Conclusion The present work shows that a native strain like F. verticillioides ITV03 using DSSB supplemented with nitrogen has a great potential as a producer of cellulase for lignocellulosic residue hydrolysis. Keywords Fusarium verticillioides ITV03 Endoglucanases b-Glucosidases Optimization Sweet sorghum bagasse Thermostability
Introduction In 2019 the global enzyme market was around $ 9.9 trillion and an annual increase of 7.1% was expected for 2020–2027 (Grand View Research 2020). Global enzyme production is led by the following companies: Novozymes (46%), Genencor now part of DuPont
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(22%), DSM (6%), and Roche, Amano, AB Enzymes, BASF, Chr. Hansen and Shin-Nihon, ADM, KAO, Verenium, Iogen, Dyadic, Meiji, Enmex, Nagase and Biozyme that all together make up 26% (Grand View Research 2020). Enmex is the main Mexican enz
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