Properties of immobilized MAS1-H108A lipase and its application in the efficient synthesis of n-3 PUFA-rich triacylglyce
- PDF / 1,623,014 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 9 Downloads / 170 Views
RESEARCH PAPER
Properties of immobilized MAS1‑H108A lipase and its application in the efficient synthesis of n‑3 PUFA‑rich triacylglycerols Xiumei Wang1,2,3 · Xiaoxu Zhao1,2 · Xiaoli Qin4 · Zexin Zhao3 · Bo Yang3 · Yonghua Wang5 Received: 20 July 2020 / Accepted: 22 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract This study reports the properties of immobilized MAS1-H108A lipase from marine Streptomyces sp. strain W007 on XAD1180 resin and its application in the synthesis of n-3 polyunsaturated fatty acids (PUFA)-rich triacylglycerols (TAG) for the first time. It was found that the optimal temperature and pH for both immobilized MAS1-H108A lipase and free lipase MAS1-H108A were 70 °C and 7.0, respectively. However, immobilized MAS1-H108A lipase exhibited higher thermostability when compared with free lipase MAS1-H108A. It was also interesting that both immobilized MAS1-H108A lipase and free lipase MAS1-H108A showed no regiospecificity in the hydrolysis of triolein. Subsequently, immobilized MAS1-H108A lipase and free lipase MAS1-H108A were employed to catalyze glycerolysis of n-3 PUFA-rich ethyl esters (EE) and esterification of n-3 PUFA with glycerol under vacuum in the solvent-free system. The results showed that n-3 PUFA-rich TAG were synthesized efficiently by non-regiospecific immobilized MAS1-H108A lipase and TAG contents separately reached 92.07% and 76.13% during the esterification and glycerolysis reactions, which were significantly higher than those (71.82% and 39.62%, respectively) obtained by free lipase MAS1-H108A. Besides, TAG exhibited similar n-3 PUFA composition to the substrate. These findings indicated that non-regiospecific immobilized MAS1-H108A lipase is a promising and efficient biocatalyst for the industrial synthesis of n-3 PUFA-rich TAG. Keywords Immobilized MAS1-H108A lipase · Triacylglycerols · n-3 polyunsaturated fatty acids · Ethyl esters · Properties
Introduction Long-chain n-3 polyunsaturated fatty acids (PUFA), particularly docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), have health benefits in the prevention and * Yonghua Wang [email protected] 1
College of Environmental and Biological Engineering, Putian University, Putian 351100, China
2
Fujian Provincial Key Laboratory of Ecology-Toxicological Effects & Control for Emerging Contaminants, Putian 351100, China
3
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China
4
College of Food Science, Southwest University, Chongqing 400715, China
5
Guangdong Research Center of Lipid Science and Applied Engineering Technology, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China
reduction of some diseases such as chronic inflammatory diseases, cardiovascular disease, and cancer [1–3]. Recent studies have shown that n-3 PUFA-rich triacylglycerols (TAG) exhibit higher bioavailability than n-3 PUFA-rich ethyl esters (EE) and are more stable than free n-3 PUFA [4, 5]. Moreover, i
Data Loading...
