Proteome of exhaled breath condensate on exposure to high-temperature thermoheliox
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Russian Chemical Bulletin, International Edition, Vol. 69, No. 9, pp. 1816—1818, September, 2020
Proteome of exhaled breath condensate on exposure to high-temperature thermoheliox S. D. Varfolomeev,a,b A. A. Panin,с A. M. Ryabokon,b,d A. S. Kozyr,b A. S. Kononikhin,b L. V. Shogenova,e and A. G. Chuchaline aInstitute
of Physicochemical Foundations of the Functioning of Neural Networks and Artificial Intelligence, M. V. Lomonosov Moscow State University, Build. 11B, 1 Leninskie Gory, 119991 Moscow, Russian Federation. Fax: +7 (499) 939 3589 bN. M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 ul. Kosygina, 119334 Moscow, Russian Federation. Fax: +7 (499) 137 4101. E-mail: [email protected] cLLC MedTechInnovations, 3-1 Blagoveshchenskii per., 123001 Moscow, Russian Federation dDepartment of Chemistry, M. V. Lomonosov Moscow State University, Build. 11B, 1 Leninskie Gory, 119991 Moscow, Russian Federation eN. I. Pirogov Russian State National Research Medical University, 1 ul. Ostrovityanova, 117997 Moscow, Russian Federation The proteome of exhaled breath condensate was analyzed by mass spectrometry before and immediately after the thermoheliox procedure and after a 3 h relaxation. The major part of the proteome remained unchanged and there was no extensive cell destruction. Key words: thermoheliox, therapy, exhaled breath condensate, proteome, mass spectrometric analysis.
The high-temperature breathing mixture of helium and oxygen (thermoheliox) started to be actively used for the treatment of various diseases, in particular, chronic obstructive pulmonary disease,1,2 ischemic stroke,3 and some pathologies of pregnancy. It is relevant to use thermoheliox for the treatment of acute viral infections, including the coronavirus infection4 and to stimulate the human immune system.5 Meanwhile, the effect of thermoheliox on the human body appears to be complex and requires additional investigations. The key question that should be answered first of all is whether or not high temperatures (60 С and above) have a significant detrimental effect on the cell structures of the respiratory system as the proteins of destroyed cells appear in the exhaled breath. In order to answer this question, we studied the composition of proteins in the human exhaled breath condensate (EBC) before and after the respiratory procedure carried out for 20 min. The inhalation temperature at the inlet of the respiratory tract was 70 С. The protein composition of the EBC after relaxation for 3 h was also studied for comparison. Experimental The exhaled breath condensate was collected by the traditional procedure using R-tube respiratory tubes (Respiratory
Research, USA).6,7 Liquid chromatography/mass spectrometry analysis (HPLC-MS/MS) was carried out using a system consisting of an Agilent 1100 chromatograph (Agilent Technologies Inc., USA) and an LTQ FT Ultra mass spectrometer (Thermo, Germany), according to previously published protocols.8 The list of exact masses of peptides and masses of their fragments was used to s
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