Proteomic analysis of human dental pulp in different clinical diagnosis
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ORIGINAL ARTICLE
Proteomic analysis of human dental pulp in different clinical diagnosis Poliana Amanda Oliveira Silva 1 & Stella Maris de Freitas Lima 2,3 & Mirna de Souza Freire 2,4 & André Melro Murad 5 & Octávio Luiz Franco 2,6 & Taia Maria Berto Rezende 1,2,3 Received: 4 May 2020 / Accepted: 27 October 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Objectives The present study aimed to identify proteins obtained from pulp tissue and correlate with each clinical diagnosis (healthy pulp, inflamed pulp, and necrotic pulp). Materials and methods A total of forty-five molars were used. Three biological replicas were evaluated. Lysis and sonication were used for protein extraction. Protein quantification was assessed by using the Bradford technique, and shotgun proteome analysis was performed by nanoUPLC-MSE using a Synapt G2 mass spectrometer. Mass spectra data were processed using the Waters PLGS software, and protein identification was done using the human Uniprot database appended to the PLGS search engine. Results A total of 123 different proteins were identified in all evaluated pulp conditions. Among these, 66 proteins were observed for healthy pulp, 66 for inflamed pulp, and 91 for necrotic pulp. Most protein identification was related to immune response, multi-organism process, platelet activation, and stress in inflamed pulp samples compared to healthy pulp. Proteins related to cellular component organization or biogenesis, developmental process, growth, immune response, multi-organism process, response to stimulus, signaling, stress, and transport were identified in cases of apical periodontitis compared to inflamed pulp. Conclusions The progression of the disease to inflamed pulp promoted a high abundance of proteins related to the immune system and stress. Comparing the necrotic pulp with inflamed pulp conditions, a high abundance of proteins was noticed related to metabolism, transport, and response between organisms. Clinical relevance This finding may assist in future studies of new markers, understanding of tissue engineering, and development of future products. Keywords Endodontics . Proteomics . Pulp . Pulpitis . Pulp necrosis
Introduction Dental pulp is a highly vascularized and innervated connective tissue closely surrounded by dentin [1, 2]. This tissue can
be exposed to inflammatory [3] and/or microbial aggressors [4]. The pulp inflammatory process triggered by these agents may vary according to the aggressors’ type and intensity [5]. With the evolution of pulp disease, innate effector cells
Supplementary Information The online version contains supplementary material available at https://doi.org/10.1007/s00784-02003660-3. * Taia Maria Berto Rezende [email protected]; [email protected] 1
Programa de Pós-graduação em Ciências da Saúde, Universidade de Brasília, Brasília, Distrito Federal, Brazil
2
Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SGAN 916N – Av. W5 – Camp
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