Proteomic Analysis of the Acid-Insoluble Fraction of Whole Saliva from Patients Affected by Different Forms of Non-hista
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ORIGINAL ARTICLE
Proteomic Analysis of the Acid-Insoluble Fraction of Whole Saliva from Patients Affected by Different Forms of Non-histaminergic Angioedema Davide Firinu 1 & Morena Arba 2 & Federica Vincenzoni 3,4 & Federica Iavarone 3,4 & Giulia Costanzo 1 & Tiziana Cabras 2 & Massimo Castagnola 5 & Irene Messana 6 & Stefano R. Del Giacco 1 & Maria T. Sanna 2 Received: 22 March 2020 / Accepted: 1 June 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract We analyzed by bidimensional electrophoresis the acid-insoluble fraction of saliva from three classes of angioedema patients and a healthy control group, highlighting significant variations of several normalized spot volumes. Characterization of the corresponding proteins was performed by in-gel tryptic digestion of the spots, followed by high-resolution HPLC-ESI-MS/MS analysis of tryptic mixtures. By this strategy, 16 differentially-expressed proteins among two or more groups were identified. We found higher concentration of proteins involved in immune response (interleukin-1 receptor antagonist and annexin A1), and of moonlighting proteins acting as plasminogen receptors (glyceraldehyde-3-phosphate dehydrogenase, α-enolase, and annexin A2) in patients affected by the idiopathic non-histaminergic or hereditary angioedema with unknown origin with respect to healthy controls. These data provide new information on the molecular basis of these less characterized types of angioedema.
Keywords 2-dimensional electrophoresis . Human saliva . Mass spectrometry . Angioedema . Tandem mass spectrometry Abbreviations AAE acquired angioedema ACN acetonitrile Abs antibodies C1-INH complement component 1 esterase inhibitor DTT Dithiotreitol EAACI European Academy of Allergy and Clinical Immunology E-FABP epidermal fatty acid-binding protein
ENOA FXII GAPDH HAWK HAE HC HMWK IAM
α-enolase, FA, formic acid coagulation factor XII glyceraldehyde-3-phosphate dehydrogenase Hereditary Angioedema International Working Group hereditary angioedema healthy controls prekallikrein-high-molecular-weight-kininogen iodoacetamide
Irene Messana, Stefano R. Del Giacco and Maria T. Sanna contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10875-020-00802-w) contains supplementary material, which is available to authorized users. * Davide Firinu [email protected] 1
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Dept of Basic Biotechnological Sciences, Intensivological and Perioperative Clinics, Catholic University of Sacred Heart, Rome, Italy
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Agostino Gemelli Foundation University Hospital IRCCS, Rome, Italy
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Proteomics Lab. IRCCS Santa Lucia Foundation, Via del Fosso di Fiorano, 64 00143 Rome, Italy
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Istituto di Scienze e Tecnologie Chimiche, “Giulio Natta” Consiglio Nazionale delle Ricerche L.go F. Vito 1, 00168 Roma, Italy
Dept of Medical Sciences and Public Health, University of Cagliari, 09042 Monserrato, CA, Italy Dept of Life and Environmental Sciences, University of Cagliari, 09042 Monserrato, CA, Italy
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