Insights from comparative proteomic analysis into degradation of phenanthrene and salt tolerance by the halophilic Marte
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Insights from comparative proteomic analysis into degradation of phenanthrene and salt tolerance by the halophilic Martelella strain AD-3 Xin Chen1 Weiwei Wang2 Haiyang Hu2 Hongzhi Tang2 Yongdi Liu1 Ping Xu2 Kuangfei Lin1 Changzheng Cui 1 ●
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Accepted: 30 October 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A halophilic PAHs-degrading strain, Martelella AD-3, was previously isolated from highly saline petroleum-contaminated soil. In this study, label-free proteomics were performed to identify differentially expressed proteins (DEPs) under Group P (phenanthrene +5% salinity) and Group G (glycerol +1% salinity), which would help to reveal the mechanism of phenanthrene degradation and salt tolerance. A total of 307 up-regulated DEPs were found in Group P, including 17 phenanthrene degradation proteins. Among these phenanthrene-degrading proteins, the ferredoxin of aromatic ring-hydroxylating dioxygenase (RHD) was up-regulated by 110-fold and gentisate 1,2-dioxygenases (GDOs) were only expressed in Group P. Besides, we also found nine high salt stress response proteins, including ectoine synthase and transport protein of compatible (osmoprotectant) solutes, were differentially up-regulated. These results indicate that strain AD-3 mainly relied on RHD and dihydrodiol dehydrogenase to degrade phenanthrene, and accumulated compatible solutes for resistance to salt stress. This study provides strong theoretical guidance for understanding the degradation of phenanthrene by strain AD-3 in high salt environments. Keywords Moderate halophilic bacteria Martelella Proteomics Degradation of phenanthrene Salt tolerance ●
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Introduction Polycyclic aromatic hydrocarbons (PAHs) are persistent organic pollutants, ubiquitous in the environment and consisting of two or more fused benzene rings (Haritash and
Supplementary information The online version of this article (https:// doi.org/10.1007/s10646-020-02310-4) contains supplementary material, which is available to authorized users. * Hongzhi Tang [email protected] * Changzheng Cui [email protected] 1
State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, People’s Republic of China
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State Key Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People’s Republic of China
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Kaushik 2009). Due to high abundance, difficult degradation, and carcinogenic effects, 16 PAHs are listed as priority pollutants by the United States and other countries (Ghosal et al. 2016; Rathour et al. 2018; Sower and Anderson 2008). PAHs pollution is closely related to the petrochemical industry, and has been detected in high-salt environments such as seawater, industrial wastewater, and saline soil in recent years (Ghosal et al. 2016; Han et al. 2020
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