Proteomic study of in vitro osteogenic differentiation of mesenchymal stem cells in high glucose condition
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ORIGINAL ARTICLE
Proteomic study of in vitro osteogenic differentiation of mesenchymal stem cells in high glucose condition Kuneerat Aswamenakul1 · Parin Klabklai1 · Supitcha Pannengpetch2 · Tulyapruek Tawonsawatruk3 · Chartchalerm Isarankura‑Na‑Ayudhya4 · Sittiruk Roytrakul5 · Chanin Nantasenamat6 · Aungkura Supokawej1 Received: 6 June 2020 / Accepted: 3 September 2020 © Springer Nature B.V. 2020
Abstract Patients with diabetes have been widely reported to be at an increased risk of secondary osteoporosis. Osteoporosis is caused by an imbalance in bone remodeling due to increased bone resorption and/or decreased osteoblast-dependent bone formation. In this study, mesenchymal stem cells (MSCs) were used as a disease model to determine the effects of high glucose levels on MSC-osteoblast development. The results indicated that under high glucose conditions, MSCs had reduced cell viability and increased number of β-galactosidase-positive cells. Furthermore, in vitro osteogenesis was shown to be reduced in MSCs cultured in osteogenic differentiation medium at 10, 25, and 40 mM glucose as demonstrated by Alizarin red S staining and alkaline phosphatase activity assay. Moreover, a proteomic study was performed in MSCs cultured with 25 and 40 mM glucose. The proteomic results demonstrated that 12 proteins were up- and downregulated in bone marrow-derived mesenchymal stem cells cultured with high glucose in a dose-dependent manner. The findings presented here contribute to our understanding of the mechanism of diabetes mellitus responsible for bone loss. However, the exact mechanism of action of hyperglycemia on bone deformability requires additional studies. Keywords High glucose · Mesenchymal stem cells · Bone formation · Proteomics
Introduction In recent years, stem cells have gained considerable interest throughout the world due to their useful and specific properties pertaining to self-renewal and multipotent differentiation [1]. Mesenchymal stem cells (MSCs) are a type of adult stem cells that have been extensively studied. Particularly, MSCs Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11033-020-05811-x) contains supplementary material, which is available to authorized users. * Aungkura Supokawej [email protected] 1
Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, 999 Phutthamonthon 4 Rd, Nakhonpathom 73170, Thailand
2
Center for Research and Innovation, Faculty of Medical Technology, Mahidol University, 999 Phutthamonthon 4 Rd, Nakhonpathom 73170, Thailand
3
Department of Orthopedics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand
have multi-differentiation potential to become adipocytes, osteocytes, and chondrocytes and can play anti-inflammatory and immunomodulatory roles [2]. The differentiation of MSCs into osteoblasts can be performed by culturing MSCs under appropriate conditions, namely, in the presence of dexamethasone, insulin and β-glycerophosphat
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