Proteomics analysis identified TPI1 as a novel biomarker for predicting recurrence of intrahepatic cholangiocarcinoma
- PDF / 1,488,175 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 85 Downloads / 218 Views
ORIGINAL ARTICLE—ALIMENTARY TRACT
Proteomics analysis identified TPI1 as a novel biomarker for predicting recurrence of intrahepatic cholangiocarcinoma Wen-Long Yu1 • Guanzhen Yu2 • Hui Dong3 • Ke Chen4 • Jun Xie5 • Hua Yu3 • Yuan Ji4 • Guang-Shun Yang6 • Ai-Jun Li7 • Wen-Ming Cong3 Guang-Zhi Jin8
•
Received: 16 January 2020 / Accepted: 13 September 2020 Ó Japanese Society of Gastroenterology 2020
Abstract Background Intrahepatic cholangiocarcinoma (ICC) is the second most common tumor in primary liver cancer, but the prognostic factors associated with long-term outcomes after surgical resection remain poorly defined. This study aimed to develop a novel prognostic classifier for patients with ICC after surgery. Methods Using a proteomics approach, we screened tumor markers that up-regulated in ICC tissues, and narrowed
down by bioinformatics analysis, western blot and immunohistochemistry. Prognostic markers were identified using Cox regression analyses in primary training cohort and the predictive models for time to recurrence (TTR) were established. The predictive accuracy of predictive model was validated in external validation cohort and prospective validation cohort. MTT assay, clonal formation assay and trans-well assays were used to verify the effect on the proliferation and migration in ICC cell line. Hua Yu [email protected]
Wen-Long Yu, Guanzhen Yu, Hui Dong, Ke Chen and Jun Xie authors contributed equally to this work.
Yuan Ji [email protected]
Ai-Jun Li, Wen-Ming Cong and Guang-Zhi Jin senior authors contributed equally to this work.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00535-020-01729-0) contains supplementary material, which is available to authorized users.
Guang-Shun Yang [email protected] 1
Department II of Biliary Tract Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai 200438, China
2
Department of Oncology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, NO.725, South Wanping RoadShanghai, Shanghai 200032, China
3
Wen-Long Yu [email protected]
Department of Pathology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai 200438, China
4
Guanzhen Yu [email protected]
Department of Pathology, Zhong Shan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China
5
Hui Dong [email protected]
The School of Life Science, Fudan University, 2005 Songhu Road, 200438, Shanghai, China
6
Department of Hepatic Surgery, Shanghai Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai 200438, China
& Ai-Jun Li [email protected] & Wen-Ming Cong [email protected] & Guang-Zhi Jin [email protected]
Ke Chen [email protected] Jun Xie [email protected]
123
J Gastroenterol
Results Triosephosphate isomerise (TPI1) was significantly up-regulated in ICC tissues and Kaplan–Meier analysis reveals that
Data Loading...
