A candidate gene for the determination of rice resistant to rice false smut
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A candidate gene for the determination of rice resistant to rice false smut Jiehua Qiu & Feifei Lu & Hong Wang & Junhui Xie & Congcong Wang & Zhiquan Liu & Shuai Meng & Huangbin Shi & Xihong Shen & Yanjun Kou
Received: 20 July 2020 / Accepted: 3 November 2020 # Springer Nature B.V. 2020
Abstract In recent years, rice false smut (RFS) has grown from being a minor to a major disease affecting rice productivity. The genetic basis of the host’s defense against RFS is currently under-researched. In this study, a rice variety Nanjing11 with stable resistance to RFS from years under naturally infected field condition was identified. The genetic analysis showed that the RFS resistance of Nanjing11 was controlled by a single dominant gene, tentatively termed as false smut resistance 1 (FSR1), which was mapped to a 220-kb interval on the long arm of chromosome 1 by using an F2 population bred from the cross Nanjing11 (RFS resistant) x CG3 (RFS susceptible). In this 220-kb region, DNMT2 (LOC_Os01g42630) was considered the most likely candidate gene for the RFS resistance of Nanjing11 on the basis of sequence variation and transcriptional responses to Ustilaginoidea virens infection. The result of this study lays the foundations for cloning and functional analysis of FSR1 and application of this gene.
Keywords Rice disease . Mapping-clone . Single nucleotide polymorphism . Ustilaginoidea virens
Jiehua Qiu and Feifei Lu contributed equally to this work. J. Qiu : F. Lu : H. Wang : J. Xie : C. Wang : Z. Liu : S. Meng : H. Shi : X. Shen (*) : Y. Kou (*) State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311401, China e-mail: [email protected] e-mail: [email protected]
Introduction Rice false smut (RFS) is a fungal disease caused by Ustilaginoidea virens (Cook) Takahashi (teleomorph form: Villosiclava virens). RFS was considered a minor disease of rice. However, with the widespread adoption of modern high-yielding cultivars and hybrids, and with the extensive use of mineral fertilizer, both the frequency of the disease and its geographical reach have spread (Ladhalakshmi et al. 2012; Qiu et al. 2019). RFS not only depresses grain yield but also downgrades grain quality as a result of the mycotoxins produced (Fan et al. 2016). At present, the RFS disease is mainly prevented by spraying fungicides at the proper time (Huang et al. 2019; Tsuda et al. 2006), and developing and cultivating resistant varieties could be a more sustainable and safe strategy to control RFS. Over the past 20 years, a substantial screening has been devoted to the identification of RFS-resistant cultivars. Previous studies have shown that there were differences of incidence of RFS disease among rice varieties, mostly susceptible. The consensus is that high-yielding hybrids and late-maturing cultivars tend to be more susceptible than early-maturing cultivars, and that japonica types are more susceptible than indica types (Fu et al. 2016; Gan et al. 2013; Huang et al. 2010; Liang et al. 2014; Liu et al. 2000; Lv et al. 20
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