A MIF haplotype is associated with the outcome of patients with severe sepsis: a case control study
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BioMed Central
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A MIF haplotype is associated with the outcome of patients with severe sepsis: a case control study Lutz E Lehmann†1, Malte Book*†1, Wolfgang Hartmann2, Stefan U Weber3, Jens-Christian Schewe3, Sven Klaschik3, Andreas Hoeft3 and Frank Stüber1 Address: 1University Department of Anaesthesiology and Pain Therapy, Inselspital, CH-3010 Bern, Switzerland, 2Department of Pathology, Bonn University, Sigmund-Freud-Str. 25, D-53105 Bonn, Germany and 3Clinic and Policlinic for Anaesthesiology and Operative Intensive Care, Bonn University, Sigmund-Freud-Str. 25, D-53105 Bonn, Germany Email: Lutz E Lehmann - [email protected]; Malte Book* - [email protected]; Wolfgang Hartmann - [email protected]; Stefan U Weber - [email protected]; Jens-Christian Schewe - [email protected]; Sven Klaschik - [email protected]; Andreas Hoeft - [email protected]; Frank Stüber - [email protected] * Corresponding author †Equal contributors
Published: 26 November 2009 Journal of Translational Medicine 2009, 7:100
doi:10.1186/1479-5876-7-100
Received: 19 June 2009 Accepted: 26 November 2009
This article is available from: http://www.translational-medicine.com/content/7/1/100 © 2009 Lehmann et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Macrophage migration inhibitory factor (MIF) plays an important regulatory role in sepsis. In the promoter region a C/G single nucleotide polymorphism (SNP) at position -173 (rs755622) and a CATT5-8 microsatellite at position -794 are related to modified promoter activity. The purpose of the study was to analyze their association with the incidence and outcome of severe sepsis. Methods: Genotype distributions and allele frequencies in 169 patients with severe sepsis, 94 healthy blood donors and 183 postoperative patients without signs of infection or inflammation were analyzed by real time PCR and Sequence analysis. All included individuals were Caucasians. Results: Genotype distribution and allele frequencies of severe sepsis patients were comparable to both control groups. However, the genotype and allele frequencies of both polymorphisms were associated significantly with the outcome of severe sepsis. The highest risk of dying from severe sepsis was detectable in patients carrying a haplotype with the alleles -173 C and CATT7 (p = 0.0005, fisher exact test, RR = 1,806, CI: 1.337 to 2.439). Conclusion: The haplotype with the combination of the -173 C allele and the -794 CATT7 allele may not serve as a marker for susceptibility to sepsis, but may help identify septic patients at risk of dying.
Background Macrophage Migration Inhibitory Factor (MIF) is a cytokine widely expressed in both immune and nonimmune cells play
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