A Novel Active Peptide from Rapana venosa Protects Against Gentamicin-Induced Sensory Hair Cell Loss in Zebrafish
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A Novel Active Peptide from Rapana venosa Protects Against Gentamicin‑Induced Sensory Hair Cell Loss in Zebrafish Yan Gao1 · Shanshan Zhang1 · Jiahui Wan2 · Rongchun Wang1 · Shuaikang Ba1 · Xiuna Ji1 · Chen Sun1 · Kechun Liu1 Accepted: 5 September 2020 © Springer Nature B.V. 2020
Abstract Gentamicin is a common aminoglycoside antibiotic used in clinical practice. However, its use is being increasingly restricted owing to its adverse effect on the inner ear. In this study, we tried to elucidate the mechanism behind the otoprotective effects exerted by a novel active peptide from Rapana venosa in zebrafish larvae. Our results showed that the combined application of gentamicin and this novel peptide helped the sensory hair cells reduce the uptake of gentamicin and subsequently restore the expression of nuclear factor erythroid-derived-2-like 2 (Nrf2), manganese superoxide dismutase (Mn-SOD), and copper/zinc superoxide dismutase (Cu/Zn-SOD). This, in turn, reduced excessive reactive oxygen species production and the incidence of apoptosis in sensory hair cells upon gentamicin administration. As a result, this peptide protected the sensory hair cells from gentamicin-induced loss. Our findings provide new insight into the application potential of an active peptide derived from marine food resources in the field of hearing protection. Keywords Active peptide · Gentamicin · Sensory hair cell · Zebrafish
Introduction Aminoglycosides (AGs) have shown effectiveness in treating multi-drug resistant infections, cystic fibrosis, tuberculosis, and urinary infections (Huth et al. 2011; Xie et al. 2011; Kyoung et al. 2016). However, it has been reported Yan Gao and Shanshan Zhang contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10989-020-10114-7) contains supplementary material, which is available to authorized users. * Chen Sun [email protected] * Kechun Liu [email protected] 1
Engineering Research Center of Zebrafish Models for Human Diseases and Drug Screening of Shandong Province, Key Laboratory for Biosensors of Shandong Province, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), 28789 Jingshidong Road, Licheng District, Jinan 250103, Shandong Province, People’s Republic of China
Shanghai Jiaotong University School of Medicine, 227 Chongqing South Road, Huangpu District, Shanghai 200025, Shanghai, People’s Republic of China
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that AGs could cause toxic side effects to the inner ear and kidney. Nephrotoxicity of AGs can be clinically managed with hydration therapy and the renal function may recover to normal (Picard et al. 2014). In contrast, the ototoxic effects caused by AGs are permanent; they damage the sensory hair cells within the mammalian inner ear, which cannot be regenerated (Esterberg et al. 2016; Leonard and Craig, 2005). The ototoxic side effects mentioned above severely limit the usage of AGs. Therefore, it is very critical to identify agents that can protect against AG-induced ototo
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