A Three-microRNA Panel in Serum: Serving as a Potential Diagnostic Biomarker for Renal Cell Carcinoma
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ORIGINAL ARTICLE
A Three-microRNA Panel in Serum: Serving as a Potential Diagnostic Biomarker for Renal Cell Carcinoma Guocheng Huang 1,2 & Xinji Li 1,2 & Zebo Chen 1 & Jingyao Wang 1 & Chunduo Zhang 1 & Xuan Chen 1,2 & Xiqi Peng 1,2 & Kaihao Liu 1,3 & Liwen Zhao 1,3 & Yongqing Lai 1,2 & Liangchao Ni 1,2 Received: 22 May 2020 / Accepted: 10 June 2020 # Arányi Lajos Foundation 2020
Abstract Purpose Renal cell carcinoma (RCC) accounts for about 120,000 death each year. Although surgery is a routine treatment, RCC could be fatal if not diagnosed at an early stage. This study aims to search for suitable serum biomarkers and construct a miRNA panel with high diagnostic sensitivity or specificity. Methods Totally 146 RCC patients and 150 normal control were involved in this three-stage study. Serum expression levels of 30 miRNAs selected from literature were tested by reverse transcription quantitative PCR (RT-qPCR) in the screening stage, the testing stage, and the validation stage. The diagnostic efficiency of miRNAs was evaluated by receiver operating characteristic (ROC) curve and area under curve (AUC) analysis. A panel with the highest diagnostic efficiency was constructed by backward stepwise logistic regression analysis. Additionally, bioinformatics analysis was used to investigate potential biological functions and mechanisms of candidate miRNAs. Results MiR-224-5p, miR-34b-3p, miR-129-2-3p and miR-182-5p with low to moderate diagnostic ability (AUC = 0.692, 0.778, 0.687 and 0.745, respectively) were selected as candidate miRNAs after the three-stage study. The final diagnostic panel was consisted by miR-224-5p, miR-34b-3p and miR-182-5p with AUC = 0.855. No significance has been found between these four miRNAs and tumor location, Fuhrman Grade and AJCC clinical stages of RCC. Bioinformatic analysis suggested that the three-miRNAs panel may participate in tumorigenesis of RCC by targeting CORO1C. Conclusions The three-miRNA panel in serum could serve as a non-invasive diagnostic biomarker of RCC. Keywords Renal cell carcinoma . Serum miRNA . Biomarker . Non-invasive
Introduction Every year, there are approximately 270,000 individuals puzzled by renal cell carcinoma (RCC) and nearly 120,000 people died because of it [1]. RCC, a radically metabolic disease, is Guocheng Huang and Xinji Li contributed equally to this work. * Yongqing Lai [email protected] * Liangchao Ni [email protected] 1
Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Guangdong 518036 Shenzhen, People’s Republic of China
2
Shantou University Medical College, Shantou, Guangdong 515041, China
3
Anhui Medical University, Hefei, Anhui 230032, China
linked to the acknowledged gene like the VHL, MET, FLCN and so on [2]. The most universal form of kidney cancer is clear-cell renal cell carcinoma which derives from the epithelium of renal tubules. Other types of kidney cancer including papillary, chromophobe, oncocytoma and collecting duct just accounts for about 25% [3]. Although n
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