A trait specific QTL survey identifies NL44, a NERICA cultivar as a novel source for reproductive stage heat stress tole
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ORIGINAL ARTICLE
A trait specific QTL survey identifies NL44, a NERICA cultivar as a novel source for reproductive stage heat stress tolerance in rice K. T. Ravikiran1 • S. Gopala Krishnan1 • K. K. Vinod1 • Gaurav Dhawan1 Priyanka Dwivedi1 • Pankaj Kumar1 • Vijay Prakash Bansal1 • M. Nagarajan2 • Prolay K. Bhowmick1 • Ranjith K. Ellur1 • Haritha Bollinedi1 • Madan Pal3 • Amitha C. R. Mithra4 • A. K. Singh1
•
Received: 7 June 2020 / Accepted: 9 November 2020 Ó Indian Society for Plant Physiology 2020
Abstract Rice is sensitive to heat stress at gametogenesis and anthesis stages. For sustaining rice yields under the predicted threat of reproductive stage heat stress (RSHS), identification of tolerant donors as well as mapping of genes governing tolerance is crucial. Recently a NERICA (NEwRIce for AfriCA) rice genotype, NL44 has been reported tolerant to RSHS. The present study aims to survey a recombinant inbred line (RIL) population developed from the cross, Pusa Basmati 1 (PB1)/NL44 using markers Electronic supplementary material The online version of this article (https://doi.org/10.1007/s40502-020-00547-z) contains supplementary material, which is available to authorized users.
linked to 54 RSHS quantitative trait loci (QTLs) through phenotypic and genotypic characterization. When exposed to RSHS, the susceptible parent PB1 and several RILs showed significant reduction for spikelet fertility and grain yield plant-1 relative to NL44. Both these traits and the estimated stress tolerance index (STI) showed a quantitative pattern of inheritance. Out of the 116 SSR markers surveyed, 31 markers were polymorphic between PB1 and NL44. No discernible associations could be found through a preliminary bulked segregant analysis with these markers. A subsequent single marker analysis revealed five minor QTLs, four for spikelet fertility under heat stress and Haritha Bollinedi [email protected]
& S. Gopala Krishnan [email protected] K. T. Ravikiran [email protected]
Madan Pal [email protected]
K. K. Vinod [email protected]
Amitha C. R. Mithra [email protected]
Gaurav Dhawan [email protected]
A. K. Singh [email protected]
Priyanka Dwivedi [email protected]
1
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
Pankaj Kumar [email protected]
2
Rice Breeding and Genetics Research Centre, ICAR-IARI, Aduthurai, Tamil Nadu 612 101, India
Vijay Prakash Bansal [email protected]
3
Division of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
M. Nagarajan [email protected]
4
ICAR- National Institute for Plant Biotechnology, New Delhi 110 012, India
Prolay K. Bhowmick [email protected] Ranjith K. Ellur [email protected]
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two for STI-spikelet fertility, of which one QTL was mapped for both the traits. These QTLs, however, could explain a very low level of total phenotypic variation. Additionally, the cumulative additive effect of these QTL
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