Abundance of Lactobacillus plantarum Strains with Beneficial Attributes in Blackberries ( Rubus sp.), Fresh Figs ( Ficus
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Abundance of Lactobacillus plantarum Strains with Beneficial Attributes in Blackberries (Rubus sp.), Fresh Figs (Ficus carica), and Prickly Pears (Opuntia ficus-indica) Grown and Harvested in Algeria Nacim Barache 1,2 & Rabia Ladjouzi 2 & Yanath Belguesmia 2 & Farida Bendali 1 & Djamel Drider 2
# Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract This first study performed on traditional fruits consumed in North Africa reveals their richness in microorganisms with beneficial attributes like cholesterol lowering capabilities. Blackberries (Rubus sp.), fresh figs (Ficus carica), and prickly pears (Opuntia ficus-indica) are fruits largely and traditionally consumed in Kabylia, a beautiful northern Algerian region. Here, 85 lactic acid bacteria (LAB)-isolates were isolated and identified by MALDI-TOF mass spectrometry. The identified species belong to Lactobacillus and Leuconostoc genera. These 85 LAB-isolates were then assessed for their capabilities to grow under conditions mimicking the gastrointestinal tract, and the resulting data were statistically treated with principal component analysis (PCA). After which, only 26 LAB-isolates were selected and characterized for their genetic relatedness using random amplified polymorphic DNA (RAPD) method. Following the genetic relatedness assessment, only 10 LAB-strains, among which nine Lactobacillus plantarum and one Lactobacillus paracasei were studied for their pathoproperties and some probiotic features. Interestingly, all of these 10 LAB-strains were devoid of adverse effects, but capable to adhere to human epithelial colorectal adenocarcinoma Caco-2 cells. Of note, these 10 LAB-strains exhibited an important in vitro hypocholesteromia effect, in straindependent manner. Moreover, the Lactobacillus strains exhibited a high bile salt hydrolase (BSH) activity which was correlated with expression of bsh2, bsh3 and bsh4 genes. Keywords Kabylian fruits . Lactobacillus . Probiotics . Caco-2 adhesion . Cholesterol lowring . bsh expression
Introduction During the last decades, consumers have progressively believed that foods are directly and efficiently implicated in health promotion [1]. Fruits and vegetables play a major role in the dietary guidance regarding their richness in Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12602-020-09632-z) contains supplementary material, which is available to authorized users. * Farida Bendali [email protected] Djamel Drider [email protected] 1
Laboratoire de Microbiologie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria
2
Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte d’Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France
micronutrients (vitamins, minerals), fibers, and a wide range of phytochemicals with human health claims [2]. Clinical and epidemiological studies revealed that fruits and vegetables consumption reduce the risks of chronic diseases [3]. In addition t
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