Ameliorative effects of sea buckthorn oil on DNCB induced atopic dermatitis model mice via regulation the balance of Th1
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(2020) 20:263
RESEARCH ARTICLE
BMC Complementary Medicine and Therapies
Open Access
Ameliorative effects of sea buckthorn oil on DNCB induced atopic dermatitis model mice via regulation the balance of Th1/Th2 Xinxin Wang1,2†, Sijia Li1†, Jiping Liu3, Dongning Kong4, Xiaowei Han5, Ping Lei5, Ming Xu5, Hongquan Guan5* and Diandong Hou5*
Abstract Background: Atopic dermatitis (AD) is a worldwide chronic skin disease which burden public health. Sea buckthorn (SBT) (Hippophae rhamnoides L., Elaeagnaceae) oil, as a traditional herbal medicine, has been used for disease treatment for many years. The effects of SBT oil on AD mouse model induced by repeated administration of 2,4dinitrochlorobenzene (DNCB) in BALB/c mice was evaluated in this study. Methods: Mice were divided into four groups including the normal control group, AD model group, AD model group treated with SBT oil (5 ml/kg) and AD model group treated with SBT oil (10 ml/kg). Same volume at different concentrations of SBT oil was applied daily on the latter two groups by gavage for 15 days following AD model induction. The function of skin barrier and the production of IL-4, IFN-γ, TNF-α and TSLP were examined after animal sacrifice. The migration and mature of langerhans cell (LCs) in lymph node was further assessed by flow cytometry. Results: SBT oil alleviated dermatitis scores, decreased ear thickness, prevented infiltration of mast cell, reduced lymph node weight and depressed activity of Th2 cells. SBT oil also reduced the expression of IL-4, IFN-γ, TNF-α and TSLP in ear tissue, IgE level in serum and mRNA relative expression of IL-4, IFN-γ, TNF-α in lymph node. Moreover, SBT oil inhibited the migration of LCs cells from local lesions to lymph node and it’s mature in lymph node. Conclusions: These results suggest SBT oil had a beneficial effect either systemic or regional on DNCB-induced AD mice via maintain the balance of Th1/Th2 and may be a potential complementary candidate for AD treatment. Keywords: Atopic dermatitis, Sea buckthorn oil, 2,4-dinitrochlorobenzene, Cytokine
Background AD is a chronic inflammatory skin disease characterized with eczematous pruritic rash which has high morbidity in children and could be recurrent in adulthood [1, 2]. As a general public health disease, the prevalence of AD has increased in recent years [3, 4]. AD affects nearly * Correspondence: [email protected]; [email protected] † Xinxin Wang and Sijia Li contributed equally to this work. 5 College of Integrated Traditional Chinese and Western Medicine, Liaoning University of Traditional Chinese Medicine, Chongshan Road NO.79, Shenyang, Liaoning 110847, P.R. China Full list of author information is available at the end of the article
20% of children and 3% of adults worldwide and the incidents become higher and higher [5]. Although the pathogenesis of AD is not explicit utterly, genetic risk, environmental factors, skin barrier dysfunction and immune dysregulation are thought to play important roles during the pathogenesis of AD [5–8]. As for immune dysreg
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