An NAC transcription factor gene from Malus baccata , MbNAC29 , increases cold and high salinity tolerance in Arabidopsi
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BIOTECHNOLOGY
An NAC transcription factor gene from Malus baccata, MbNAC29, increases cold and high salinity tolerance in Arabidopsis Deguo Han 1 & Man Du 1 & Zhengyi Zhou 1 & Shuang Wang 1 & Tiemei Li 1 & Jiaxin Han 1 & Tianlong Xu 1 & Guohui Yang 1 Received: 20 April 2020 / Accepted: 8 July 2020 / Editor: Yong Eui Choi # The Society for In Vitro Biology 2020
Abstract NAC (no apical meristem [NAM], Arabidopsis thaliana transcription activation factor [ATAF1/2], and cup-shaped cotyledon [CUC2]) TFs are involved in stress responses in plants. However, their roles in abiotic stresses are still not well known in Malus plants. In the present study, MbNAC29 from Malus baccata was identified and characterized. Quantitative real-time PCR analysis revealed that the expression of MbNAC29 was induced by cold, high salinity, drought, and heat. The open reading frame (ORF) of MbNAC29 is 1122 bp, encodes 373 amino acids. Subcellular localization showed that the MbNAC29-GFP (green fluorescent protein) fusion protein was localized in the nucleus. Furthermore, MbNAC29 was highly expressed in new leaf and mature leaf. When MbNAC29 was introduced into Arabidopsis, it improved cold and high salinity tolerance in transgenic plants under lowtemperature stress (− 4°C) and salt stress (200 mM NaCl). When MbNAC29 was introduced into Arabidopsis, it increased the survival rate of transgenic lines than wild type (WT) under cold and high-salinity stresses. In addition, overexpression of MbNAC29 in transgenic Arabidopsis increased the contents of chlorophyll and proline and the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), while decreased the content of malondialdehyde (MDA). Therefore, these results suggest that MbNAC29 probably plays an important role in response to cold and salt stresses in Arabidopsis by enhancing scavenging capability of reactive oxygen species (ROS). Keywords Malus baccata . MbNAC29 . Cold stress . Salt stress
Introduction Apple, as one of the most important economic forest tree species, is widely grown in the world, but in recent years, due to the frequent occurrence of frost, the yield and quality of apples in some producing areas have been seriously affected (Yang et al. 2015a). Malus baccata (L.) Borkh, as one of the main Malus plants, is widely used as an apple rootstock in northern China, and also as a source of forestry wood or greening tree species. Due to its high resistance to low temperature and drought, M. baccata is also used as a breeding
* Tiemei Li [email protected] * Guohui Yang [email protected] 1
The Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin 150030, China
material for cold and drought resistance (Han et al. 2020b). In addition to low-temperature stress, abiotic stresses such as drought and high salinity are also the main limiting factors affecting the geographical distri
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