Analysis of cell growth dynamics of Pediococcus acidilactici in the presence of inulin in an optimized microenvironment
- PDF / 1,465,423 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 104 Downloads / 158 Views
ORIGINAL PAPER
Analysis of cell growth dynamics of Pediococcus acidilactici in the presence of inulin in an optimized microenvironment Sharmistha Samanta Koruri1 · Ranjana Chowdhury1 · Pinaki Bhattacharya2
Received: 30 March 2015 / Revised: 21 May 2015 / Accepted: 22 June 2015 / Published online: 10 July 2015 © Springer-Verlag Berlin Heidelberg 2015
Abstract The present investigation deals with the optimization of cell growth rate of the candidate probiotic Pediococcus acidilactici in the presence of the specific prebiotic inulin. Three independent variables viz. concentration of inulin, concentration of glucose and pH have been selected for optimization study using response surface methodology. Theoretical analysis indicates that the maximum cell growth rate occurs at pH 7, 20 g/dm3 concentration of inulin and 20 g/dm3 concentration of glucose. Validation of these values has been done through a set of programmed experiments. Studies on cell dynamics in the presence of different concentrations of inulin have also been carried out to identify any limitation on the initial inulin concentration. Results clearly indicate that cell growth is enhanced with the increase in inulin concentration. However, there is a critical value of the prebiotic concentration (20 g/dm3 inulin) beyond which the cell growth is inhibited. A summative type growth model has been proposed to explain the growth behaviour of P. acidilactici in the presence of the dual substrate, i.e. glucose and inulin. While growth on glucose follows Monod model, Haldane-type substrate-inhibited growth model holds good for growth on inulin. Intrinsic kinetic parameters for all the model equations have been determined experimentally. Keywords Probiotic P. acidilactici · Prebiotic inulin · Growth kinetics · RSM Communicated by Erko Stackebrandt. * Ranjana Chowdhury [email protected] 1
Chemical Engineering Department, Jadavpur University, Kolkata 700032, India
2
Department of Chemical Engineering, Heritage Institute of Technology, Kolkata 700107, India
Introduction Recently, combinations of probiotics and prebiotics are aiming towards a more targeted development of functional dietary supplement and for better understanding of the metabolism of prebiotics by probiotics. This is leading modern research facilitating rational development of synbiotics. Although a range of probiotic–prebiotics mixtures are available commercially, the targets for enhancement of the efficiency of the synbiotics are lacking. Factors determining the systematic understanding of synergy between prebiotics and beneficial probiotics are a challenging topic for modern research. Inulin has been recognized as one of the most preferred prebiotics for its synergistic effect on the growth of probiotic microorganisms in general. In nature, inulin is abundantly available in many plant species such as Liliaceae, Gramineae, Amaryllidaceae, Compositae (Franck and Leenheer 2000). Most of these plants are grown in Asian subcontinents and other tropical countries. Although the use of inulin
Data Loading...
