Anti-adipogenic effect of Lactobacillus fermentum MG4231 and MG4244 through AMPK pathway in 3T3-L1 preadipocytes
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Anti-adipogenic effect of Lactobacillus fermentum MG4231 and MG4244 through AMPK pathway in 3T3-L1 preadipocytes SukJin Kim1 • Soo-Im Choi1 • Miran Jang3 • Yulah Jeong4 • Chang-Ho Kang4 Gun-Hee Kim1,2
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Received: 24 April 2020 / Revised: 20 August 2020 / Accepted: 25 August 2020 / Published online: 6 October 2020 Ó The Korean Society of Food Science and Technology 2020
Abstract This study evaluated the anti-adipogenic effects and mechanisms underlying the action of Lactobacillus fermentum MG4231 and MG4244 strains on adipogenesis and lipid accumulation in 3T3-L1 preadipocytes. Treatment with cell-free extracts (CFEs) from the two strains reduced lipid accumulation and intracellular triglyceride production in 3T3-L1 adipocytes by more than 50%. The inhibitory effects of L. fermentum on lipid accumulation were mediated by the downregulation of FAS and aP2 resulting from the inhibition of PPARc and C/EBPa gene expression. Moreover, AMPK and HSL phosphorylation was upregulated by CFE treatment. These results indicated that the anti-adipogenic and lipolysis activities of L. fermentum strains were caused by increased AMPK and HSL & Gun-Hee Kim [email protected] SukJin Kim [email protected] Soo-Im Choi [email protected] Miran Jang [email protected] Yulah Jeong [email protected] Chang-Ho Kang [email protected]
phosphorylation. Both strains displayed high leucine arylamidase and b-galactosidase enzymatic activity, with excellent adhesion to epithelial cells. Therefore, we identified L. fermentum as potential new probiotics for the prevention of obesity. Keywords L. fermentum MG4231 MG4244 Adipogenesis Probiotics Abbreviations CFE Cell-free extract DEX Dexamethasone IBMX Isobutylmethylxanthine DMEM Dulbecco modified Eagle’s medium FBS Fetal bovine serum TG Triglyceride MTT Tetrazolium bromide salt PPARc Peroxisome proliferator-activated receptor c C/EBPa CCAAT/enhancer-binding protein a FAS Fatty acid synthase aP2 Adipose-specific fatty acid-binding protein SREBP1 Sterol regulatory element-binding protein 1 LPL Lipoprotein lipase ACC Acetyl CoA carboxylase AMPK AMP-activated protein kinase p-AMPK Phospho-AMP-activated protein kinase HSL Hormone-sensitive lipase p-HSL Phospho-hormone- sensitive lipase
1
Department of Bio-Health Convergence Major, Duksung Women’s University, Seoul 01369, Republic of Korea
2
Department of Food and Nutrition, Duksung Women’s University, Seoul 01369, Republic of Korea
Introduction
3
Department of Food Science, Purdue University, West Lafayette, IN 47906, USA
4
MEDIOGEN, Co., Ltd., Jecheon 27159, Republic of Korea
Obesity is caused by excessive energy accumulation and metabolic imbalance. Public awareness regarding the problem of obesity is rising, but the number of overweight
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or obese patients continues to increase worldwide (Blu¨her, 2019). Obesity is associated with several factors, including eating habits, lack of activity, stress, and endocrine system changes. Obesity is considered a key risk factor for serious metabolic diseases, such as t
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