Anti-inflammatory activities of puerarin in high-fat diet-fed rats with streptozotocin-induced gestational diabetes mell
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ORIGINAL ARTICLE
Anti‑inflammatory activities of puerarin in high‑fat diet‑fed rats with streptozotocin‑induced gestational diabetes mellitus Wenting Xu1 · Mengyu Tang1 · Jiahui Wang1 · Lihong Wang1 Received: 27 May 2020 / Accepted: 4 September 2020 © The Author(s) 2020
Abstract To investigate the effect of puerarin on insulin resistance and inflammation in rats with gestational diabetes mellitus (GDM). Gestational diabetic model rats were established by intraperitoneal injection of streptozotocin (25 mg/kg) combined with high-fat feeding and were randomly assigned to three groups: the control group, the GDM group, and the puerarin-treated group. Puerarin was intragastrically administered to rats daily until the offspring were born. The rats in both the GDM group and control group were administered the same volume of normal saline. Serum total cholesterol, triglycerides, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol in all groups of rats were measured. Haematoxylin and eosin staining was used to evaluate morphological changes in the liver, pancreas, and adipose tissues around the reproductive organs. Western blotting was carried out to measure the protein expression of IRS-1 and inflammatory factors, including TNF-α, TLR4, MyD88 and phosphorylated NF-κB, in the adipose tissues around the reproductive organs. Puerarin had preventive effects on GDM-induced pathological changes and ameliorated glucose and lipid metabolism disorders in GDM rats. Puerarin upregulated IRS-1 expression and decreased the protein expression of TNF-α, TLR4, and MyD88 as well as the levels of phosphorylated NF-κB in adipose tissues around the reproductive organs in GDM rats. This study indicated that puerarin exerts anti-inflammatory effects by downregulating the important TLR4/MyD88/NF-κB inflammatory signalling pathway. Therefore, puerarin can decrease the expression of TNF-α and ameliorate insulin resistance in GDM rats, suggesting the potential efficacy of puerarin in GDM treatment. Keywords Puerarin · Gestational diabetes mellitus (GDM) · Signalling pathway · IRS-1 Abbreviations GDM Gestational diabetes mellitus IR Insulin resistance STZ Streptozotocin TRL4 Toll-like receptor 4 MyD88 Myeloid differentiation primary response gene 88 NF-κB Nuclear factor-κB * Lihong Wang [email protected] Wenting Xu [email protected] Mengyu Tang [email protected] Jiahui Wang [email protected] 1
Department of Reproduction, Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Zhang jiagang, Suzhou, Jiangsu, China
FBG Fasting blood glucose FINS Fasting blood insulin TC Total cholesterol TG Triglycerides HDL-C High-density lipoprotein cholesterol LDL-C Low-density lipoprotein cholesterol STZ Streptozotocin
Introduction Gestational diabetes mellitus (GDM) refers to hyperglycaemia in pregnant women with no prior history of diabetes mellitus. It has been reported that the morbidity of GDM is 5–10% and has increased significantly in recent years [1]. Studies rep
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