Anti-inflammatory Activity of Mollugin on DSS-induced Colitis in Mice
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Anti-inflammatory Activity of Mollugin on DSS-induced Colitis in Mice* Juan LI1, Jin-ling ZHANG2, Xue-peng GONG1, Meng XIAO1, Yuan-yuan SONG1, Hui-fang PI3, Guang DU1# 1 Department of Pharmacy, Tongji Hospital , Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China 2 Department of Pharmacy, The Central Hospital of Xianning, Xianning 437100, China 3 School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China Huazhong University of Science and Technology 2020
Summary: We aimed to explore the anti-inflammatory activity of mollugin extracted from Rubia cordifolia L, a traditional Chinese medicine, on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice. Thirty C57BL/6 mice were divided into a control group (n=6), a model group (n=6), and three experimental groups (40, 20, 10 mg/kg of mollugin, n=6 each). DSS solution (3%) was given to mice in the model group and experimental groups from day 4 to day 10 to induce the mouse UC model. Mice in the experimental groups were intragastrically administrated mollugin from day 1 to day 10. Animals were orally given distilled water in the control group for the whole experiment time and in the model group from day 1 to day 3. The changes in colon pathology were detected by hematoxylin and eosin (HE) staining. Interleukin-1β (IL-1β) in the serum, and tumor necrosis factor-α (TNF-α) and interferon-γ (IFN) in the tissues were measured by enzyme linked immunosorbent assay. Expression levels of Toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 in the colon tissues were detected by immunohistochemistry. Results showed that mollugin could significantly reduce weight loss and the disease activity index in the DSS-induced UC mouse model. HE examinations demonstrated that mollugin treatment effectively improved the histological damage (P
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