Antiinflammatory properties of antiLy6G antibody disappear during magnetic field exposure in rats with carrageenan induc
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ORIGINAL ARTICLE
Antiinflammatory properties of antiLy6G antibody disappear during magnetic field exposure in rats with carrageenan induced acute paw inflammation Tufan Mert 1
&
Tuba Ozcan Metin 2 & Mehmet Sahin 3 & Selma Yaman 4
Received: 28 April 2020 / Accepted: 17 June 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Antiinflammatory properties of pulsed magnetic field (PMF) treatments or administration of antiLy6G antibody have been previously reported. In this study, we hypothesized that, the combination of PMF treatments and antiLy6G administration may synergistically potentiate their antiinflammatory actions. The effects of the combination of PMF treatments and antiLy6G administration were investigated by examining the inflammatory signs, histopathological properties of the inflamed site, and measuring the macrophage inflammatory protein-1 alpha (MIP-1α/CCL3) and myeloperoxidase (MPO) levels of inflamed paw tissues in rats with carrageenan-induced acute paw inflammation. In this present study, PMF treatments alone or administration of antiLy6G alone ameliorated the acute inflammation. However, their combination exacerbated the inflammatory signs, hyperalgesia, allodynia, edema and fever, and aggravated the inflammatory conditions by excessive infiltration of inflammatory cells to the inflamed site. These opposing effects of the combined treatments may correlate with enhanced levels of MIP-1α and MPO in inflamed paws. Present results indicated that the combination of the PMF treatments and antiLy6G administration may not provide additional benefits and may actually cause an aggravation of the acute inflammatory process. Findings may also suggest that during neutrophil or immune cell-targeted treatments for inflammatory states, magnetic field exposure may cause unexpected negative consequences. Keywords Pulsed magnetic field . AntiLy6G antibody . MIP-1α . Inflammation . Rats
Introduction * Tufan Mert [email protected]; [email protected] Tuba Ozcan Metin [email protected] Mehmet Sahin [email protected] Selma Yaman [email protected] 1
Department of Biophysics, Faculty of Medicine, Bolu Abant Izzet Baysal University, 14030 Bolu, Turkey
2
Department of Histology and Embryology, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey
3
Department of Medical Biology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey
4
Department of Biophysics, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey
Inflammation is the body’s immunological defense mechanism against any tissue damage, injury or invading bacterial and viral pathogens (Medzhitov 2008). Inflammatory process also plays important roles in the development of various pathophysiologies, including rheumatoid arthritis, multiple sclerosis, diabetes, cancer, cardiovascular, and respiratory diseases (Ashley et al. 2012). Acute inflammatory process includes activation and migration of immune-inflammatory cells and the release of various medi
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