Assessment of myocardial infarction in mice by Late Gadolinium Enhancement MR imaging using an inversion recovery pulse
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Assessment of myocardial infarction in mice by Late Gadolinium Enhancement MR imaging using an inversion recovery pulse sequence at 9.4T Catherine Chapon1, Amy H Herlihy2 and Kishore K Bhakoo*1 Address: 1Stem Cell Imaging, MRC Clinical Sciences Centre, Hammersmith Hospital, Imperial College London, UK and 2Biological Imaging Centre, MRC Clinical Sciences Centre, Hammersmith Hospital, Imperial College London, UK Email: Catherine Chapon - [email protected]; Amy H Herlihy - [email protected]; Kishore K Bhakoo* - [email protected] * Corresponding author
Published: 24 January 2008 Journal of Cardiovascular Magnetic Resonance 2008, 10:6
doi:10.1186/1532-429X-10-6
Received: 20 December 2007 Accepted: 24 January 2008
This article is available from: http://www.jcmr-online.com/content/10/1/6 © 2008 Chapon et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Purpose: To demonstrate the feasibility of using an inversion recovery pulse sequence and to define the optimal inversion time (TI) to assess myocardial infarction in mice by late gadolinium enhancement (LGE) MRI at 9.4T, and to obtain the maximal contrast between the infarcted and the viable myocardium. Methods: MRI was performed at 9.4T in mice, two days after induction of myocardial infarction (n = 4). For cardiovascular MR imaging, a segmented magnetization-prepared fast low angle shot (MP-FLASH) sequence was used with varied TIs ranging from 40 to 420 ms following administration of gadolinium-DTPA at 0.6 mmol/kg. Contrast-to-noise (CNR) and signal-to-noise ratio (SNR) were measured and compared for each myocardial region of interest (ROI). Results: The optimal TI, which corresponded to a minimum SNR in the normal myocardium, was 268 ms ± 27.3. The SNR in the viable myocardium was significantly different from that found in the infarcted myocardium (17.2 ± 2.4 vs 82.1 ± 10.8; p = 0.006) leading to a maximal relative SI (Signal Intensity) between those two areas (344.9 ± 60.4). Conclusion: Despite the rapid heart rate in mice, our study demonstrates that LGE MRI can be performed at 9.4T using a protocol similar to the one used for clinical MR diagnosis of myocardial infarction.
Introduction Late Gadolinium Enhancement (LGE) cardiovascular magnetic resonance (CMR) imaging using a T1-weighted sequence with an inversion recovery (IR) pre-pulse is commonly used for the clinical diagnosis of myocardial infarction in humans [1,2], and experimentally in large animals [3]. The time to inversion (TI) is selected to null the signal intensity (SI) in the non-infarcted (viable) myo-
cardium, following administration of chelated gadolinium, in order to increase contrast between the viable and hyper-enhanced infarcted myocardium [1]. Late gadolinium
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