Assessment of oncogenic role of intestinal microbiota in colorectal cancer patients
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ORIGINAL RESEARCH
Assessment of oncogenic role of intestinal microbiota in colorectal cancer patients Tha’ir Abid D’asheesh 1 & Bashdar Mahmud Hussen 2 & Ali H. Al-Marzoqi 3 & Abdolmajid Ghasemian 4 Accepted: 27 September 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Introduction The direct association between some microbial species and cancers, such as in colorectal cancer (CRC), has been disclosed. Objective The aim of this study was to evaluate the changes in intestinal microbiota in subjects with CRC compared with healthy group. Methods Three-hundred fecal specimens were gathered from patients with CRC and 300 from healthy individuals during March 2014 to October 2019 from two hospitals in Tehran. The informed consent form and the questionnaire were completed by the patients. Following the identification of Lactobaccilus acidophilus (L. acidophilus), Lactobacillus palntarom (L. palntarom), and Enterococcus faecalis (E. faecalis), the number of bacteria was determined using quantitative real-time PCR (qPCR). Results The patients’ age range was 20–76 years (mean: 55.34 ± 3.66). The qPCR clarified that number of E. faecalis was 2.2fold higher in patients with CRC compared to healthy population (p = 0.0013). Additionally, the number of L. acidophilus and L. plantarom was 3.4-fold (p < 0.0001) and 4.8-fold (p < 0.0001) higher in healthy population. Conclusion The inhibitory effect of intestinal microflora against the CRC development was proposed by observation of the changes in intestinal microbiota and determining their composition in subjects with CRC compared with that of healthy individuals. Microbiota was considered as a goal for the prevention and treatment of CRC. The relationship between microbiota and human health would be known deeper; this knowledge provides insights into the management of intestinal microbiota and therapeutics. Keywords Lactobacillus spp . Enterococcus faecalis . colorectal cancer
Introduction Colorectal cancer (CRC) is a major outstanding cancer among countries with an exacerbating status in recent years [1, 2]. Environmental or epigenetic reasons play a higher role than genetic factors for the CRC predisposition. The primary * Abdolmajid Ghasemian [email protected] 1
Department of Environment, College of Science, University of Al-Qadisiyah, Diwaniyah, Iraq
2
Department of Pharmacognosy, College of Pharmacy, Hawler Medical University, Erbil, Iraq
3
Department of Biology, College of Science for Women, University of Babylon, Hillah, Iraq
4
Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
crucial prognostic indicator of the CRC development includes appearance of appendages called polyps in the epithelial layer of the colon. Recent research has outlined that timely detection of colorectal polyps in the early stages using screening and removal techniques are critical for CRC prevention and reduction of the associated mortality [3]. Adenomas are the most common and eminent pathologic types of colon pol
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