Bacterial Invasiveness
Intracellular pathogens are responsible for a number of important diseases worldwide, including tuberculosis, plague and bacillary dysentery. This volume focusses on those intracellular pathogens that have been studied most extensively at the molecular, g
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Editors R.W. Compans, Atlanta/Georgia M. Cooper, Birmingham/Alabama· H. Koprowski, Philadelphia/Pennsylvania· F. Melchers, Basel M. Oldstone, La Jolla/California· S. Olsnes, Oslo M. Potter, Bethesda/Maryland· H. Saedler, Cologne P.K. Vogt, La Jolla/California· H. Wagner, Munich
Springer Berlin Heidelberg New York Barcelona Budapest Hong Kong London Milan Paris Santa Clara Singapore Tokyo
Bacterial Invasiveness Edited by V.L. Miller
With 16 Figures
Springer
Professor V,RG,N,A L. MiLLER, Ph.D. Department of Microbiology and Molecular Genetics University of California, Los Angeles 405 Hilgard Avenue. Los Angeles CA 90095, USA
Cover illustration: The Legionnaires' disease bacterium, Legionella pneumophila, has an intracellular fate that is determined by the icmA locus. Upon infection, wildtype L. pneumophila Philadelphia-I is placed in a unique phagosome that does not fuse with host Iysosomes. Therefore, phagosomes containing fluoresceinated wild-type L. pneumophila (green) show no colocalization with host Iysosomes prelabelled with rhodamine-dextran (red) in macrophage-like U937 cells (Figure 1). In contrast, L.pneumophila mutant 250 contains an icmA mutation and can not prevent phagosome-lysosome fusion. Digestion of fluoresceinated mutant bacteria within phagolysosomes and dispersion of its contents generate many punctated yellow vesicles containing the colocalized bacterial and lysosomal markers (Figure 2). For both images, macrophage-like U937 cells were incubated for 36 hours with the lysosomal marker lysine-fixable rhodamine-dextran (70,000 MW), washed and then infected with L. pneumophila that had been previously labelled by a succinimidyl ester of fluorescein. After incubating 6 hours for the wild-type and 16 hours for the mutant L-pneumophila, the cells were fixed and imaged by fluorescent confocal microscopy. Confocal fluorescent images by Lawrence A. Wiater.
Cover design: Kunke/+Lopka, IIvesheim ISBN-13: 978-3-642-85218-3
e-ISBN-13: 978-3-642-85216-9
001: 10.1007/978-3-642-85216-9 This work is subject to copyright. All rights are reserved. whether the whole or part ofthe material is concerned. specifically the rights of translation. reprinting. reuse of illustrations. recitation, broadcasting. reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965. in its current version. and permission for use must always be obtained from Springer-Verlag. Violations are liable for prosecution under the German Copyright Law.
© Springer-Verlag Berlin Heidelberg 1996 Library of Congress Catalog Card Number 15-12910
Softcover reprint ofthe hardcover 1st edition 1996 The use of general descriptive names. registered names, trademarks. etc. in this publication does not imply. even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Product liability: The publisher
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