Cadaverine reverse transporter (CadB protein) contributes to the virulence of Aeromonas veronii TH0426
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ORIGINAL ARTICLE
Cadaverine reverse transporter (CadB protein) contributes to the virulence of Aeromonas veronii TH0426 LuoTao Tao 1 & YuanHuan Kang 1 & Lei Zhang 1 & Qiumei Shi 2 & Ying Li 1 & Tonglei Wu 2 & Aidong Qian 1 & WuWen Sun 1 Xiaofeng Shan 1
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Received: 8 July 2019 / Revised: 6 January 2020 / Accepted: 9 January 2020 # Springer Nature Switzerland AG 2020
Abstract Aeromonas veronii is one of the main pathogens causing sepsis and ulcer syndrome in freshwater fish. Analysis of the results of epidemiological investigations in recent years has revealed that the virulence of A. veronii and its tolerance to drugs have been increasing year by year. Currently, most of the research on A. veronii focuses on its isolation, identification, and drug susceptibility, whereas research on its virulence factors and pathogenesis mechanisms is relatively rare. In this study, we identified and obtained the highly expressed TH0426 cadaverine reverse transporter (CadB) of A. veronii. We used efficient suicide plasmidmediated homologous recombination to delete the cadB gene in TH0426 and constructed a cadB deletion strain. The LD50 of ΔcadB was 93.2 times higher than that of TH0426 in zebrafish, the toxicity of ΔcadB was 9.5 times less than that of TH0426 in EPC cells, and the biofilm formation ability of ΔcadB was 5.6-fold greater than that of TH0426. In addition, motility detection results indicated that ΔcadB had lost its swimming ability. The results of flagellar staining and TEM demonstrated that ΔcadB shed the flagella. In summary, the virulence and adhesion of A. veronii TH0426 were significantly decreased by the deletion of cadB, which might provide a theoretical basis for research into A. veronii virulence factors. Keywords Aeromonas veronii TH0426 . CadB . Virulence
Introduction Aeromonas veronii, an emerging pathogen of aquatic organisms, is also important in food safety and public health (Chen et al., 2015; Song et al., 2018; Chandrarathna et al., 2018; Ku & Yu, 2017; Chen et al., 2013). Currently, A. veronii can be isolated from almost all environments (Khalifa & Bekhet, 2018; Hassan et al., 2017; Samie et al., 2012; Nawaz et al., 2010), and its prevalence in food and the environment might be an indirect cause of human illness (Shiina et al., 2004; LuoTao Tao and YuanHuan Kang contributed equally to this study. * WuWen Sun [email protected] * Xiaofeng Shan [email protected] 1
College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, Jilin, China
2
Key Laboratory of Hebei Province Preventive Veterinary Medicine, Hebei Normal University of Science & Technology, Qinhuangdao 066004, China
Wang et al., 2002). A. veronii has many infection routes (Abdelhamed et al., 2019), which cause specific difficulties for related research. The patient population infected by A. veronii has gradually increased, and the pathogen has shown a trend of growing virulence and drug resistance year by year. An increasing number of countries have recognised the hazards of A. veronii and listed it as an
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