Cellulose Derivatives Enhanced Stability of Alginate-Based Beads Loaded with Lactobacillus plantarum LAB12 against Low p
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Cellulose Derivatives Enhanced Stability of Alginate-Based Beads Loaded with Lactobacillus plantarum LAB12 against Low pH, High Temperature and Prolonged Storage Ismail M. Fareez 1,2 & Siong Meng Lim 1,2 & Nurul Aida Ashyqin Zulkefli 1 & Rakesh K. Mishra 1 & Kalavathy Ramasamy 1,2
# Springer Science+Business Media New York 2017
Abstract The susceptibility of probiotics to low pH and high temperature has limited their use as nutraceuticals. In this study, enhanced protection of probiotics via microencapsulation was achieved. Lactobacillus plantarum LAB12 were immobilised within polymeric matrix comprised of alginate (Alg) with supplementation of cellulose derivatives (methylcellulose (MC), sodium carboxymethyl cellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC)). L. plantarum LAB12 encapsulated in Alg-HPMC(1.0) and Alg-MC(1.0) elicited improved survivability (91%) in simulated gastric conditions and facilitated maximal release (∼100%) in simulated intestinal condition. AlgHPMC(1.0) and Alg-MC(1.0) significantly reduced (P < 0.05) the viability loss of LAB12 (viability loss 9 log CFU g−1). These findings strongly suggest the potential of cellulose derivatives supplemented Alg bead as protective micro-transport for probiotic strains. They can be safely incorporated into new functional food or nutraceutical products. * Kalavathy Ramasamy [email protected] 1
Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), 42300 Bandar Puncak Alam, Selangor Darul Ehsan, Malaysia
2
Collaborative Drug Discovery Research (CDDR) Group, Pharmaceutical and Life Sciences Community of Research, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor Darul Ehsan, Malaysia
Keywords Microencapsulation . Probiotic lactobacilli . Alginate . Cellulosic derivatives
Introduction The large intestine of a healthy adult contains approximately 1011 bacteria of varying species per milliliter of colonic content [1]. It is well known that modifications of the gut microbiota balance through ingestion of probiotic-based products could provide health benefits to the host. Probiotics, which are Blive microorganisms which when administered in adequate amounts confer health benefits to the host^ [2], promote growth of good gut microbiota, inhibit pathogens and stimulate host’s immune response [3]. Health benefits, which have been attributed to probiotic consumption, range from alleviations of lactose intolerance to increasing resistance to gut infections and suppression of colon cancer [4]. In spite of their beneficial effects, the introduction of probiotics into manufactured products (functional food or pharmaceuticals) remains challenging. Sufficient numbers of live bacteria cells, allegedly more than 108 colony-forming units (CFU) per serving or 106 to 107 CFU g−1, have been indicated as the essential dose to confer beneficial effects to the host [5]. As such, probiotics need to survive the harsh condition of the upper gastrointestinal tract in order to colonise the gut in appropriate population. Unfortunately, the number of live microorg
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