Characterization of Sll1558 in environmental stress tolerance of Synechocystis sp. PCC 6803
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ORIGINAL ARTICLE
Characterization of Sll1558 in environmental stress tolerance of Synechocystis sp. PCC 6803 Junji Uchiyama1,2 · Yutaro Ito3 · Ayumi Matsuhashi2 · Yuta Ichikawa2 · Mamoru Sambe2 · Shuichi Kitayama2 · Yuka Yoshino2 · Atushi Moriyama2 · Hidetaka Kohga2 · Satoru Ogawa4 · Hisataka Ohta1,2 Received: 28 November 2019 / Accepted: 11 May 2020 © Springer Nature B.V. 2020
Abstract So far, the molecular mechanisms underlying the acidic-stress responses of plants are complicated and only fragmentally understood. Here, we investigated the mechanisms responsible for acidic-stress acclimation. Previously, DNA microarray analysis identified the sll1558 gene in Synechocystis sp. PCC 6803 (hereafter called Synechocystis 6803) to be upregulated following short-term acid treatment (1 h at pH 3.0). The sll1558 gene encodes uridine diphosphate–glucose pyrophosphorylase (UDP-glucose pyrophosphorylase), which catalyzes the conversion of glucose-1-phosphate into UDP-glucose. We constructed mutant cells for this gene and analyzed their phenotype. The sll1558 gene did not completely segregate in sll1558 mutant cells; thus, Sll1558 is essential for the survival of Synechocystis 6803. Besides, the partially disrupted sll1558 mutant cells were highly sensitive to acidic stress (pH 6.0) as well as other stress conditions (high salt, high osmolality, high/low temperature, and ultraviolet-B stress); the number of sll1558 transcripts increased under these conditions. UDP-glucose is used for the synthesis of various materials, such as glycolipids. From the membrane lipid composition analysis, digalactosyldiacylglycerol decreased and phosphatidylglycerol increased in the partially disrupted sll1558 mutant cells under acidic stress. These results suggest that sll1558 is important not only for the survival of Synechocystis 6803, but also for tolerance under various stress conditions. Keywords Acidic stress · Cyanobacteria · Uridine diphosphate-glucose pyrophosphorylase · Environmental stress
Department of Biology, Faculty of Science, Tokyo University of Science, Shinjuku‑ku, Tokyo 162‑8601, Japan
2
MGDG Monogalactosyldiacylglycerol OD Optical density OM Outer membrane PG Phosphatidylglycerol qRT-PCR Quantitative reverse transcription polymerase chain reaction SDS-PAGE Sodium dodecyl sulfate–polyacrylamide gel electrophoresis Synechocystis 6803 Synechocystis sp. PCC 6803 SQDG Sulfoquinovosyldiacylglycerols TEM Transmission electron microscopy UDP Uridine diphosphate UV Ultraviolet WT Wild type
Graduate School of Mathematics and Science Education, Tokyo University of Science, Shinjuku‑ku, Tokyo 162‑8601, Japan
3
Department of Biological Science and Technology, Tokyo University of Science, Katsushika‑ku, Tokyo 125‑8585, Japan
Introduction
Abbreviations CPSs Capsular polysaccharides DGDG Digalactosyldiacylglycerol EPS Exopolysaccharide LPSs Lipopolysaccharides Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11120-020-00759-2) contains supplementary material,
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