Clinical relevance of Neutral Endopeptidase (NEP/CD10) in melanoma
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Clinical relevance of Neutral Endopeptidase (NEP/CD10) in melanoma Elsa F Velazquez†1,6, Molly Yancovitz†1, Anna Pavlick1,2, Russell Berman3, Richard Shapiro3, Dusan Bogunovic2, David O'Neill2, Yi-Lo Yu2, Joanna Spira1, Paul J Christos4, Xi Kathy Zhou4, Madhu Mazumdar4, David M Nanus5, Leonard Liebes2, Nina Bhardwaj1,2, David Polsky1 and Iman Osman*†1,2 Address: 1Ronald O. Perelman Department of Dermatology, New York University School of Medicine, New York, NY, USA, 2Department of Medicine, New York University School of Medicine, New York, NY, USA, 3Department of Surgery, New York University School of Medicine, New York, NY, USA, 4Department of Public Health, Weill Medical College of Cornell University, New York, NY, USA, 5Department of Medicine, Weill Medical College of Cornell University, New York, NY, USA and 6Department of Pathology, Brigham and Women's Hospital, Harvard School of Medicine, Boston, MA, USA Email: Elsa F Velazquez - [email protected]; Molly Yancovitz - [email protected]; Anna Pavlick - [email protected]; Russell Berman - [email protected]; Richard Shapiro - [email protected]; Dusan Bogunovic - [email protected]; David O'Neill - [email protected]; Yi-Lo Yu - [email protected]; Joanna Spira - [email protected]; Paul J Christos - [email protected]; Xi Kathy Zhou - [email protected]; Madhu Mazumdar - [email protected]; David M Nanus - [email protected]; Leonard Liebes - [email protected]; Nina Bhardwaj - [email protected]; David Polsky - [email protected]; Iman Osman* - [email protected] * Corresponding author †Equal contributors Published: 05 January 2007 Journal of Translational Medicine 2007, 5:2
doi:10.1186/1479-5876-5-2
Received: 11 August 2006 Accepted: 05 January 2007
This article is available from: http://www.translational-medicine.com/content/5/1/2 © 2007 Velazquez et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Overexpression of Neutral Endopeptidase (NEP) has been reported in metastatic carcinomas, implicating NEP in tumor progression and suggesting a role for NEP inhibitors in its treatment. We investigated the role of NEP expression in the clinical progression of cutaneous melanoma. Methods: We screened 7 melanoma cell lines for NEP protein expression. NEP-specific siRNA was transfected into the lines to examine the role of gene transcription in NEP expression. Immunohistochemistry was done for 93 specimens and correlated with clinicopathologic parameters. Thirty-seven metastatic melanoma specimens were examined for NEP transcript expression using Affymetrix GeneChips. In a subset of 25 specimens for which both transcript and protein expression was available
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