CMV pp65 and IE-1 T cell epitopes recognized by healthy subjects
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BioMed Central
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CMV pp65 and IE-1 T cell epitopes recognized by healthy subjects Stefanie L Slezak, Maria Bettinotti, Silvia Selleri, Sharon Adams, Francesco M Marincola and David F Stroncek* Address: Department of Transfusion Medicine, Warren G. Magnuson Clinical Center National Institutes of Health, Bethesda, Maryland, USA Email: Stefanie L Slezak - [email protected]; Maria Bettinotti - [email protected]; Silvia Selleri - [email protected]; Sharon Adams - [email protected]; Francesco M Marincola - [email protected]; David F Stroncek* - [email protected] * Corresponding author
Published: 28 March 2007 Journal of Translational Medicine 2007, 5:17
doi:10.1186/1479-5876-5-17
Received: 9 January 2007 Accepted: 28 March 2007
This article is available from: http://www.translational-medicine.com/content/5/1/17 © 2007 Slezak et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Adoptive immune and vaccine therapies have been used to prevent cytomegalovirus (CMV) disease in recipients of hematopoietic progenitor cell transplants, but the nature of T cell responses to CMV have not been completely characterized. Methods: Peptide pools and individual peptides derived from the immune-dominant CMV proteins pp65 and IE-1 and antigen-specific, cytokine flow cytometry were used to characterize the prevalence and frequency of CD4+ and CD8+ memory T cells in 20 healthy CMV-seropositive subjects. Results: CD8+ T cell responses to pp65 were detected in 35% of subjects and to IE-1 in 40% of subjects. CD4+ T cell responses to pp65 were detected in 50% of subjects, but none were detected to IE-1. Several new IE-1 HLA class I epitopes were identified, including 4 restricted to HLA-C antigens. One region of IE-1 spanning amino acids 300 to 327 was rich in class I epitopes. The HLA class I restrictions of IE-1 peptides were more promiscuous than those of pp65 peptides. Conclusion: Since naturally occurring CD4+ and CD8+ T cell responses to pp65 were detectable in many subjects, but only CD8+ T cell responses to IE-1 were detected, pp65 may be better than IE-1 for use in vaccine and adoptive immune therapies.
Background Cytomegalovirus (CMV) is a persistent virus in normal hosts in which primary infection is typically controlled through a combination of adaptive and innate immune responses. Viral latency follows primary infection and continues for life, usually without major symptoms. Although 60% to 80% of adults that test seropositive for CMV antibodies show no symptoms of infection, in immunocompromised hosts, such as patients undergoing hematopoietic stem cell transplantation (HSCT) or recipients of organ transplants, CMV can cause severe dis-
ease[1]. In the past 10 years, the incidence of CMV disease in
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