Complete genome sequence of the original Taiwanese isolate of sweet potato latent virus and its relationship to other po
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ANNOTATED SEQUENCE RECORD
Complete genome sequence of the original Taiwanese isolate of sweet potato latent virus and its relationship to other potyviruses infecting sweet potato Mingqiang Wang • Jorge Abad • Segundo Fuentes Ruhui Li
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Received: 23 January 2013 / Accepted: 23 March 2013 Ó Springer-Verlag Wien (outside the USA) 2013
Abstract The complete genome of sweet potato latent virus (SPLV) was determined to be 10081 nucleotides long excluding the 3’ poly (A) tail. The genome contains a single large open reading frame encoding a polyprotein of 3247 amino acids. Its genomic organization is typical of potyviruses and contains motifs conserved in members of the genus Potyvirus. Pairwise comparisons show that SPLV shares identities of 50.0 %-56.3 % to other potyviruses at the genomic sequence level. Phylogenetic analysis shows that SPLV is closely related to four other sweet potato potyviruses in the sweet potato feathery mottle virus lineage, but it lacks the unique PISPO in the P1 region of those viruses. The genome analyses confirm that SPLV is a distinct sweet potato virus in the genus Potyvirus.
Members of six species of the genus Potyvirus infect sweet potato and cause significant yield losses in sweet potato production worldwide [1]. Sweet potato latent virus (SPLV), formerly designated as sweet potato virus N, was first reported from Taiwan [2]. The virus was then found in several other countries in Asia and Africa [3–6]. SPLV has flexuous, filamentous particles that are approximately 700-750 nm long, and it induces typical cylindrical
M. Wang R. Li (&) USDA-ARS, National Germplasm Resources Laboratory, Beltsville, MD 20705, USA e-mail: [email protected] J. Abad Plant Germplasm Quarantine Program, USDA-APHIS-PPQPHP, Beltsville, MD 20705, USA S. Fuentes International Potato Center (CIP), Lima, Peru
inclusions in the cytoplasm of infected cells. The experimental host range of SPLV is wider than that of sweet potato feathery mottle virus (SPFMV), inducing symptoms on some Chenopodium and Nicotiana species [2, 7]. Similar to SPFMV and sweet potato virus G (SPVG), a single infection by SPLV is symptomless in most sweet potato cultivars, but co-infection with sweet potato chlorotic stunt virus (SPCSV) causes synergistic disease [8]. SPLV is serologically related to, but distinct from, SPFMV [7]. Sequence comparison of 3’-partial sequences showed that SPLV is a member of a distinct species in the genus Potyvirus [9]. In this study, the complete genomic sequence of SPLV was determined, and phylogenetic analysis clearly indicated that SPLV is a distinct potyvirus that is most closely related to sweet potato virus 2 (SPV2) and other viruses in the SPFMV lineage. The SPLV isolate originally collected from a diseased sweet potato cv. Tainung 63 co-infected with SPFMV in Taiwan in 1970s [2] was used in this study. The infected sweet potato plant was transferred from Taiwan to the International Potato Center (CIP) in 1980s. SPLV was transmitted to Nicotiana clevelandii by mechanical inoculation and maintain
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