Complete genome sequence of a new achyranthes virus A isolate from Achyranthes bidentata in China
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Complete genome sequence of a new achyranthes virus A isolate from Achyranthes bidentata in China Xinyang Wu1,2 · Yuchao Lai2 · Lanqing Lv2 · Kelei Han2 · Ziqiang Chen2 · Yuwen Lu2 · Jiejun Peng2 · Lin Lin2 · Jianping Chen1,2 · Hongying Zheng2 · Fei Yan2 Received: 9 July 2020 / Accepted: 8 September 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract We have determined the complete genomic sequence of a potyvirus from Achyranthes bidentata in Zhejiang, China, using reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The genomic RNA is 9482 nucleotides (nt) long excluding the 3′-terminal poly(A) tail and encodes a putative large polyprotein with 3073 amino acids (aa). It has 75.4–53.5% nt sequence identity and 84.0–49.1% polyprotein sequence identity to other potyviruses and is probably a distantly related isolate of the same species as the recently reported achyranthes virus A isolate from South Korea (AcVA-SK). This is the first report of the occurrence of a potyvirus infecting A. bidentata in China. Achyranthes bidentata is a herbaceous perennial that grows on the edges of hillsides, in forests, and on riverbanks in many parts of Asia, Central Africa, Southern Africa, and Latin America. It has medicinal properties and has been included in official Chinese Pharmacopoeias [1]. The viruses that have been reported so far from this plant are the potexvirus alternanthera mosaic virus, the fabavirus broad bean wilt virus 2 (BBWV-2), and the newly reported potyvirus achyranthes virus A (AcVA) [2–4]. During 2018-2020, mosaic and crinkle symptoms were observed on the leaves of A. bidentata growing in Ningbo city, Zhejiang province, China (Fig. 1a). In electron microscopy of negatively stained crude Handling Editor: Stephen John Wylie. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04839-5) contains supplementary material, which is available to authorized users. * Hongying Zheng [email protected] * Fei Yan [email protected] 1
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro‑products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China
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leaf sap of two symptomatic plants, flexuous filaments about 700-900 nm in length were observed (Fig. 1b), suggesting the possible presence of a potyvirus, carlavirus, or potexvirus. Total RNA was extracted from symptomatic leaves using an EASYspin RNA Plant Mini Kit (Aidlab Biotechnologies Co., Ltd, China) and reverse transcribed using the primer M4T or a random primer and a First Strand cDNA Synthesis Kit (Toyobo, Osaka, Japan). Tests were then done to detect the presence of potyviruses, carlaviruses, potexviruses, or BBWV-2 by RT-PCR using previously reported
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