Construction of a sensitive and specific lead biosensor using a genetically engineered bacterial system with a luciferas
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RESEARCH
BioMedical Engineering OnLine Open Access
Construction of a sensitive and specific lead biosensor using a genetically engineered bacterial system with a luciferase gene reporter controlled by pbr and cadA promoters Esmail Nourmohammadi1,2, Saman Hosseinkhani3, Reza Nedaeinia4 , Hoda Khoshdel‑Sarkarizi2, Mozhdeh Nedaeinia5, Maryam Ranjbar6, Neshat Ebrahimi7, Zahra Farjami1, Mohammad Nourmohammadi8, Ali Mahmoudi1, Mohammad Goli9, Gordon A. Ferns10 and Majid Sadeghizadeh11* *Correspondence: [email protected] 11 Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Full list of author information is available at the end of the article
Abstract Background: A bacterial biosensor refers to genetically engineered bacteria that produce an assessable signal in the presence of a physical or chemical agent in the environment. Methods: We have designed and evaluated a bacterial biosensor expressing a lucif‑ erase reporter gene controlled by pbr and cadA promoters in Cupriavidus metallidurans (previously termed Ralstonia metallidurans) containing the CH34 and pI258 plasmids of Staphylococcus aureus, respectively, and that can be used for the detection of heavy metals. In the present study, we have produced and evaluated biosensor plasmids designated pGL3-luc/pbr biosensor and pGL3-luc/cad biosensor, that were based on the expression of luc+ and under the control of the cad promoter and the cadC gene of S. aureus plasmid pI258 and pbr promoter and pbrR gene from plasmid pMOL30 of Cupriavidus metallidurans. Results: We found that the pGL3-luc/pbr biosensor may be used to measure lead concentrations between 1–100 μM in the presence of other metals, including zinc, cadmium, tin and nickel. The latter metals did not result in any significant signal. The pGL3-luc/cad biosensor could detect lead concentrations between 10 nM to 10 μM. Conclusions: This biosensor was found to be specific for measuring lead ions in both environmental and biological samples. Keywords: Lead, Bacterial biosensor, Pbr promoter, Luciferase, cadA promoter
Background Ecological heavy metal pollution is a common problem that can lead to damage to human health [1]. These heavy metal pollutants may lead to environment damage and harmful ecological outcomes [2], and hence the development of sensitive, efficient, rapid and cost-effective methods is necessary to screen for the presence of these harmful
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