Contents of Myelin Basic Protein and Autoantibodies against Brain Proteins in the Experimental Antiphospholipid Syndrome
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Neurophysiology, Vol. 52, No. 2, March, 2020
Contents of Myelin Basic Protein and Autoantibodies against Brain Proteins in the Experimental Antiphospholipid Syndrome O. Z. Yaremchuk1 Received November 18, 2019 We examined the content of autoantibodies against brain proteins, content of myelin basic protein (MBP), and level of NO synthesis in the cerebellum and cerebral hemispheres on day 18 of pregnancy in BALB/c mice with the experimental antiphospholipid syndrome (APS); the effects of L-arginine on the above indices were also evaluated. As was found, the contents of autoantibodies against brain proteins having the molecular masses 120, 150, and > 170 kDa were greater than in the control. Under APS conditions, the amount of eNOS-produced NO was relatively insufficient; this was observed against the background of total hyperproduction of NO synthesized by iNOS in blood serum. In APS mice, the contents of stable NO metabolites, NO 2– and NO 3–, in the cerebellum were higher, while these levels in the cerebral hemispheres were lower with respect to the control. There were reasons to believe that the effects of L-arginine under APS conditions of and cerebral dysfunction are provided not only at the expense of influences upon the NO system, but also via antioxidant and cytoprotective properties of this amino acid. In pregnant APS mice, the content of MBP (95–110 kDa) in the cerebellum and that of MBP (18.4 kDa) in the cerebral hemispheres were greater than in the control. In APS animals, administration of L-arginine provided increase in the content of MBP (18.4 kDa) in the cerebral hemispheres compared with the respective index with no treatment. Our results show that the remyelination processes in animals with the APS are activated; this may be interpreted as a compensatory response to the injury.
Keywords: antiphospholipid syndrome, myelin basic protein (MBP), autoantibodies, nitric oxide, cerebellum, cerebral hemispheres, pregnant BALB/c mice.
INTRODUCTION The antiphospholipid syndrome (APS) is an autoimmune disease characterized by the presence of antiphospholipid antibodies (aPL) and glycoproteins linked with the above phospholipids in the blood. The APS is characterized by recurrent arterial and/or venous thromboses in the vessels of different dimensions and location, interruption of pregnancy, thrombocytopenia, and also by neurological, cardiovascular, and other disorders [1, 2]. Among patients with repetitive miscarriage, the APS is observed in 27–42% of the cases. With no adequate treatment, the embryonic death occurs in 90–95% of pregnant women with the presence of aPL [3]. In such a situation, the attention of researchers was attracted by determination of
1 Horbachevsky Ternopil National Medical University, Ternopil, Ukraine Correspondence should be addressed to O. Z. Yaremchuk (e-mail: [email protected]).
antibodies to cardiolipin, phosphatidylserine, and phosphoinositol. An increase in the level of antibodies against β2-glycoprotein-I is the most reliable symptom in the organism of patients with t
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