Contribution of the mitogen-activated protein kinase Hog1 to the halotolerance of the marine yeast Debaryomyces hansenii
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ORIGINAL ARTICLE
Contribution of the mitogen‑activated protein kinase Hog1 to the halotolerance of the marine yeast Debaryomyces hansenii Norma Silvia Sánchez1 · Martha Calahorra1 · James González2 · Tatiana Defosse3 · Nicolas Papon3 · Antonio Peña1 · Roberto Coria1 Received: 5 June 2020 / Revised: 10 July 2020 / Accepted: 17 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Halotolerant species are adapted to dealing continually with hyperosmotic environments, having evolved strategies that are uncommon in other organisms. The HOG pathway is the master system that regulates the cellular adaptation under these conditions; nevertheless, apart from the importance of Debaryomyces hansenii as an organism representative of the halotolerant class, its HOG1 pathway has been poorly studied, due to the difficulty of applying conventional recombinant DNA technology. Here we describe for the first time the phenotypic characterisation of a null HOG1 mutant of D. hansenii. Dhhog1Δ strain was found moderately resistant to 1 M NaCl and sensitive to higher concentrations. Under hyperosmotic shock, DhHog1 fully upregulated transcription of DhSTL1 and partially upregulated that of DhGPD1. High osmotic stress lead to long-term inner glycerol accumulation that was partially dependent on DhHog1. These observations indicated that the HOG pathway is required for survival under high external osmolarity but dispensable under low and mid-osmotic conditions. It was also found that DhHog1 can regulate response to alkali stress during hyperosmotic conditions and that it plays a role in oxidative and endoplasmic reticulum stress. Taken together, these results provide new insight into the contribution of this MAPK in halotolerance of this yeast. Keywords HOG pathway · Glycerol · Transcription regulation · Stl1 symporter · Gpd1 · Alkali-halotolerant · Oxidative stress · ER stress
Introduction Communicated by M. Kupiec. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00294-020-01099-3) contains supplementary material, which is available to authorized users. * Norma Silvia Sánchez [email protected] * Roberto Coria [email protected] 1
Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 México City, CDMX, México
2
Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México. Ciudad Universitaria, 04510 México City, CDMX, México
3
Groupe d’Etude des Interactions Hôte‑Pathogène, GEIHP, EA 3142, SFR ICAT 4208, UNIV Angers, UNIV Brest, Institut de Biologie en Santé, IRIS, CHU Angers, Angers, France
Debaryomyces hansenii (anamorph: Candida famata) is a halotolerant yeast able to grow in the presence of high NaCl concentrations. This yeast can tolerate as much as 4 Μ of salt, while the growth of the budding yeast Saccharomyces cerevisiae is completely inhibited with 1.7 Μ NaCl (Onishi 1963; Prista et al. 2016). Originally isolated f
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