Corms as a source of explants for the successful clonal propagation of Crocus cancellatus
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J. Crop Sci. Biotech. 2012 (March) 15 (1) : 47 ~ 51 DOI No. 10.1007/s12892-011-0113-4 RESEARCH ARTICLE
Corms as a Source of Explants for the Successful Clonal Propagation of Crocus cancellatus Mahdi Ahouran1*, Ramin Hosseini1, Reza Zarghami2 Department of Agricultural Biotechnology Engineering, College of Engineering and Technology, Imam Khomeini International University, Qazvin, Iran 2 Department of Tissue culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
1
Received: December 1, 2011 / Revised: January 2, 2012 / Accepted: February 6, 2012 Ⓒ Korean Society of Crop Science and Springer 2012
Abstract The genus Crocus comprises plants with a potential to be developed as a new ornamental crop but to date, there are not many reports on in vitro propagation of many members of this genus. The present study involves in vitro propagation of Crocus cancellatus with ornamental and horticultural value. Two different types of corm explants (apical and basal halves of corms) were cultivated onto Murashige and Skoog’s (MS) medium supplemented with different levels of α-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). One to five cormlets emerged from every responding explant through direct organogenesis. Apical halves of corms were more highly responsive than basal halves and produced a maximum multiplication rate with 3.45 ± 0.06 cormlets per explant in 95.33 ± 2.33% of the explants in MS medium supplemented with 3% sucrose and 2 mg L-1 NAA and 1 mg L-1 BAP. The effect of cold storage temperature on in vitro cormlets sprouting was studied. Cormlets stored at 4°C for 8 weeks had more statistically significant positive effects on cormlets sprouting from the controls. In vitro rooting of cormlets was induced on MS medium without plant hormones. Key words: cormlet, Iridaceae, organogenesis, propagation
Introduction Iridaceae is a great and diverse family with about 65 genera, approximately 2,025 species with a wide range of uses, but many hundreds of these attractive species have been ignored for developing new horticultural crops (Ascough et al. 2009; Niederwieser et al. 2002). Crocus belongs to the family of Iridaceae, is a hardy perennial that produce inflorescences with brightly colored flowers, and has great potential for development as ornamental plants in parks and gardens. It is estimated that the Crocus which attracts many people because of their colorful flowers will have a high economic value in the near future (Kandemir 2010). Crocus is not only a delightful addition to the garden, but also is an important traditional medicinal and culinary herb as well. Some studies have demonstrated antitumor, antimutagenic and cytotoxMahdi Ahouran ( ) E-mail :[email protected] Tel : +98-919-302-88-39 / Fax : +98-21-6659-39-96 Present address : Faroogh Life Sciences Research Laboratory, No: 96 Parcham St., Tohid Sq., Tehran, Iran The Korean Society of Crop Science
ic activities, and inhibitory effects of the Crocus extract on the intracellular nucleic aci
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