COS-7 cells are a cellular model to monitor polyomavirus JC miR-J1-5p expression
- PDF / 480,914 Bytes
- 5 Pages / 595.276 x 790.866 pts Page_size
- 3 Downloads / 130 Views
SHORT COMMUNICATION
COS-7 cells are a cellular model to monitor polyomavirus JC miR-J1-5p expression Simone Agostini1 · Roberta Mancuso1 · Andrea Saul Costa1 · Franca Rosa Guerini1 · Mario Clerici1,2 Received: 27 July 2020 / Revised: 2 September 2020 / Accepted: 25 September 2020 © Springer Nature B.V. 2020
Abstract Polyomavirus JC (JCPyV) is a ubiquitous human neurotropic virus that can cause progressive multifocal leukoencephalopathy (PML), sometimes as a consequence of drug treatment for disabling diseases, including Multiple Sclerosis. JCPyV expresses microRNAs (miRNAs), and in particular miR-J1-5p, but at now we have limited knowledge regarding this aspect. In the present study the expression of JCPyV miR-J1-5p was measured in infected COS-7, to verify if and when this miRNA is expressed in a cell model of JCPyV-MAD-4 strain infection. Results showed that miR-J1-5p expression was relatively constant inside the cells from 11 days to 35 days after infection (mean: 4.13 × 105 copies/μg), and became measurable in supernatants 18 days after infection (mean: 7.20 × 104 copies/μl). miR-J1-5p expression in supernatants peaked (3.76 × 105 copies/μl) 25 days after infection and started to decrease 32 days after infection (7.20 × 104 copies/μl). These data show that COS-7 cells, already used as model for JCPyV replication cycle, can be also utilized to study JCPyV miRNAs expression, potentially opening new research avenues for diseases in which current therapeutic approaches could result in severe adverse effects (e.g. Natalizumab-associated JCPyV reactivation in Multiple Sclerosis patients). In these situations monitoring of miR-J1-5p may shed light on the mechanisms of virus reactivation and may help the clarification of the mechanisms responsible for such severe side effects. Keywords JCPyV · COS-7 · ddPCR · Multiple sclerosis · Rehabilitation
Introduction Polyomavirus JC (JCPyV) is a ubiquitous human virus belonging to the Polyomavirus family, which was isolated for the first time in 1971 [1], and can infect human oligodendrocytes, astrocytes, kidney epithelial cells and peripheral blood cells [2, 3]. JCPyV is the etiological agent of progressive multifocal leukoencephalopathy (PML), a rare but often fatal demyelinating disease of the central nervous system for which no therapeutic and/or rehabilitative treatments are available [4]. Polyomavirus JC is a circular non-enveloped doublestrand DNA virus, with a relatively small genome (about 5000 bp) [5]. Its genome is organized in two different * Simone Agostini [email protected] 1
IRCCS Don Carlo Gnocchi Foundation - ONLUS, P.zza Morandi, 3, 20100 Milan, Italy
Department of Pathophysiology and Transplantation, University of Milan, via Francesco Sforza 35, Milan, Italy
2
regions of similar size, known as early and late transcript units. These units are divided by a non-coding control region (NCCR) containing the origin of DNA replication (ori), the TATA box, cellular transcription factors binding sites and enhancer elements for the transcriptio
Data Loading...
