Cytological and genetic characterisation of dominant GMS line Shaan-GMS in Brassica napus L.
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PLANT GENETICS • ORIGINAL PAPER
Cytological and genetic characterisation of dominant GMS line Shaan-GMS in Brassica napus L. Xiaojuan Zhang 1,2,3 & Haiyan Chen 1,4,5 & Qian Zhang 1,4 & Yunxiao Zhang 1,4 & Zhaodi Xiao 1,4 & Yuan Guo 1,4 & Fei Yu 1,2 & Shengwu Hu 1,4 Received: 11 December 2019 / Revised: 13 June 2020 / Accepted: 17 June 2020 # Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2020
Abstract Genic male sterility (GMS) is an effective pollination control system applied in the hybrid breeding of Brassica napus L. ShaanGMS is a spontaneous mutant of dominant GMS in B. napus. In this research, anther abortion in the homozygous two-type line 9A15AB derived from Shaan-GMS was characterised with the combined use of light microscopy and transmission electron microscopy. Results indicated that the most striking differences between the fertile and sterile plants occurred in the tapetum in the early microsporocyte stage. In sterile plants, the tapetal cells were irregularly arranged, multi-layered and occupied the growing space of microsporocytes. When entering into meiosis, the tapetum cells degraded and the cytoplasm fused. Some oval monolayer or bilayer membrane organelles existed in the tapetal cells in sterile anthers. Mitochondria in the tapetal cells were abnormal, and middle layer cells degraded early. Pollen mother cells of Shaan-GMS degenerated at the start of meiosis and ceased at the anaphase I stage, with no dyads or tetrads formed. The combined effects of the abnormal development of the tapetum, the middle layer cells and meiosis lead to male sterility in Shaan-GMS. Inheritance of male sterility of Shaan-GMS is controlled by a monogenically multiallelic locus with three different alleles (Ms, ms and Mf), with a relationship expressed as Mf > Ms and Ms > ms. The findings help lay the foundation for illustrating the mechanism of male sterility and the utilisation of Shaan-GMS in rapeseed. Keywords Brassica napus L. . Dominant genic male sterility . Meiosis . Microsporogenesis . Tapetum development
Introduction Dominant genic male sterility (GMS) is a phenomenon wherein half of the plants in each generation are sterile when the
sterile plants in this kind of GMS line are pollinated with fertile plants within the same species. However, further studies have indicated that in some cases when a male sterile genotype is crossed with a fertile genotype of the same species, the
Xiaojuan Zhang and Haiyan Chen contributed equally to this work. Communicated by: Izabela Pawłowicz Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13353-020-00570-8) contains supplementary material, which is available to authorized users. * Fei Yu [email protected]
3
School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, Shaanxi, People’s Republic of China
4
College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, People’s Republic of China
5
College of Animal Science and Technology, Northwest A&F Univers
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