Cytoprotective Effect of Echinochrome A in Primary Culture of Pulmonary Fibroblasts from Albino Rats under Conditions of
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Cell Technologies in Biology and Medicine, No. 2, August, 2020
Cytoprotective Effect of Echinochrome A in Primary Culture of Pulmonary Fibroblasts from Albino Rats under Conditions of Oxidative Stress E. N. Sazonova1,2, M. S. Kuznetsova1, E. A. Vasileva3, N. P. Mishchenko3, N. A. Tsimbalist2, and О.А. Lebed’ko1,2 Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 125-128, June, 2020 Original article submitted December 12, 2019 We studied the effect of echinochrome A on the primary culture of pulmonary fibroblasts under normal conditions and under oxidative stress. Exposure to echinochrome A (100 μM, 6 h) did not affect the production of superoxide radical by fibroblasts, area of their nuclei, and number of nucleoli, but reduced the total area of nucleolar organizer regions against the background of stable proliferative activity of the culture. Two-hour oxidative stress induced by hydrogen peroxide solution (60 μM) increased the generation of superoxide radical, decreased DNAsynthetic activity of fibroblasts, area of fibroblast nuclei, and total area of nucleolar organizer regions. Preliminary administration of echinochrome A significantly attenuated the damaging effect of oxidative stress: the intensity of production of superoxide radicals decreased, DNAsynthetic activity and nucleus area of fibroblasts partially recovered; normalization of the total area of nucleoli was accompanied by an increase in their number. Key Words: oxidative stress; fibroblasts; echinochrome A Echinochrome A (2,3,5,6,8-pentahydroxy-7-ethyl-1,4naphtoquinone, Ech A), the active substance of Histochrome preparation [14], is a marine bioproduct extracted from sea urchins Scaphechinus mirabilis [13] and exhibiting various pharmacological effects: antioxidant [16,25], anti-inflammatory [17], immunomodulating [8], antifibrotic [12], negative inotropic [24], and anticholinesterase [26] effects. The efficiency of Ech A against tick-borne encephalitis viruses and herpes simplex type 1 viruses was reported [19]. Ech A is an atypical C-iota kinase protein inhibitor and dose-dependently stimulates differentiation of embryonic stem cells into cardiomyocytes [23]. Ech A-based drugs are used in cardiology [3,6] and ophthalmology [15,18] and are effective in the treatment of patients Khabarovsk Branch of Far-Eastern Research Center of Physiology and Pathology of Breath, Research Institute of Maternity and Childhood Protection, Khabarovsk, Russia; 2Far Eastern State Medical University, Ministry of Health of the Russian Federation, Khabarovsk; 3G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Division of Russian Academy of Sciences, Vladivostok, Russia. Address for correspondence: [email protected]. O. A. Lebed’ko 1
with destructive changes in the respiratory system [79] and with gastroduodenal ulcers [1,2]. Diversity of Ech A effects is difficult to explain by exclusively its antioxidant and chelating properties; therefore, attention to molecular-cellular mechanism of the effects of ECA is growing. Recent studies
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