Different loci control resistance to different isolates of the same race of Colletotrichum lindemuthianum in common bean
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ORIGINAL ARTICLE
Different loci control resistance to different isolates of the same race of Colletotrichum lindemuthianum in common bean Larissa Carvalho Costa1 · Rafael Storto Nalin2 · Mariana Andrade Dias1 · Márcio Elias Ferreira3 · Qijian Song4 · Marcial A. Pastor‑Corrales4 · Oscar P. Hurtado‑Gonzales4,5 · Elaine Aparecida de Souza1 Received: 14 February 2020 / Accepted: 17 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message Linkage and genome-wide association analyses using high-throughput SNP genotyping revealed different loci controlling resistance to different isolates of race 65 of Colletotrichum lindemuthianum in common bean. Abstract Development of varieties with durable resistance to anthracnose is a major challenge in common bean breeding programs because of the extensive virulence diversity of Colletotrichum lindemuthianum fungus. We used linkage and genome-wide association analyses to tap the genomic regions associated with resistance to different isolates of race 65. Linkage mapping was done using an F 2 population derived from the cross between the Mesoamerican common beans BRS Estilo x Ouro Vermelho, inoculated with two different isolates of race 65. Association genetics relied on a diversity common bean panel containing 189 common bean accessions inoculated with five different isolates of race 65 as an attempt to validate the linkage analysis findings and, eventually, identify other genomic regions associated with resistance to race 65. The F2 population and diversity panel were genotyped with the BARCBean6K_3 Illumina BeadChip containing 5398 SNP markers. Both linkage and genome-wide association analyses identified different loci controlling resistance to different isolates of race 65 on linkage group Pv04. Genome-wide association analysis also detected loci on Pv05, Pv10 and Pv11 associated with resistance to race 65. These findings indicate that resistance to race 65 can be overcome by the virulence diversity among different isolates of the same race and could lead to the loss of resistance after cultivar release. We identified 25 resistant common bean cultivars to all five isolates of race 65 in the diversity panel. The accessions should be useful to develop cultivars combining different resistance genes that favor durable resistance to anthracnose in common bean. Keywords Colletotrichum lindemuthianum · Race 65 · Mapping of resistance genes · Phaseolus vulgaris · Plant breeding · Association mapping Communicated by Janila Pasupuleti. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00122-020-03713-x) contains supplementary material, which is available to authorized users. * Elaine Aparecida de Souza [email protected] 1
Department of Biology, Universidade Federal de Lavras, Lavras, Minas Gerais, Brazil
2
Department of Genetics, Escola Superior de Agricultura Luiz de Queiroz, Piracicaba, São Paulo, Brazil
3
Plant Genetics Laboratory, EMBRAPA–Genetic Resources and Biotechnology, Br
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