Diversity in self-organized forms and migration modes in isolated epithelial cells
- PDF / 1,650,608 Bytes
- 6 Pages / 595.276 x 790.866 pts Page_size
- 51 Downloads / 159 Views
ORIGINAL ARTICLE
Diversity in self‑organized forms and migration modes in isolated epithelial cells Shota Mise1 · Shimon Shibagaki1 · Seiya Nishikawa1 · Hiroko Nakamura2 · Hiroshi Kimura2 · Atsuko Takamatsu1 Received: 2 April 2020 / Accepted: 13 September 2020 / Published online: 13 October 2020 © International Society of Artificial Life and Robotics (ISAROB) 2020
Abstract It is widely believed that cells, derived from different species or different cell lines, behave differently. However, this study reports that a variety of forms and migration modes in isolated epithelial cells of Madin–Darby Canine Kidney type were observed, although the cells were taken from the same cell line and the experimental conditions were kept constant. To understand the diverse formation processes in such cell behavior, a simple mathematical model, namely the particle-fiber model, was constructed. In this model, a single cell is assumed to be composed of a multiple of particles, interconnected by stress fibers. The particles mimic focal adhesion biding to a substrate. The stress fibers mimic a cytoskeleton, that plays an important role in maintaining the shape and the movement of the cell. Here, a growth process was introduced, which varied the size of the particles and the thickness of the fibers in dependence on the forces exerted on the particles. Simulation of the results showed that various cell shapes can be self-organized even if the parameters, which describe cell properties and their interactions with environment, were kept constant. Keywords Cell shape · Cell movement · Epithelial cells · MDCK · Particle model
1 Introduction Isolated cells, derived from different species or different cell lines, usually show different types of formation and migration modes. Some examples are a mouse fibroblast exhibiting a back-and-forth transformation between a spindle shape and a spherical one [3], fish keratocytes exhibiting a straight traveling motion, while maintaining a D-shape [7], Dictyostelium discoideum exhibiting random locomotion with complex shapes [8]. These examples suggest the cell behavior is determined genetically. In contrast, by observing isolated epithelial cells, the Madin-Darby Canine Kidney (MDCK) S. Mise, S. Shibagaki and S. Nishikawa are equally contributed. This work was presented in part at the 3rd International Symposium on Swarm Behavior and Bio-Inspired Robotics (Okinawa, Japan, November 20–22, 2019). * Atsuko Takamatsu [email protected] 1
Department of Electrical Engineering and Bioscience, Waseda University, Tokyo, Japan
Department of Mechanical Engineering, Tokai University, Kanagawa, Japan
2
cells, and by using a simple mathematical model description, we show that even cells, derived from the same cell line and under constant environmental conditions, can exhibit a variety of cell forms and migration modes.
2 Observation of the behavior of isolated epithelial cells MDCK cells are well known as model systems for tissue formation accompanied by cell migration. Examples of such systems are found
Data Loading...
