Effect of CMC Concentration on Cell Growth Behavior of PVA/CMC Hydrogel
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Article www.springer.com/13233 pISSN 1598-5032 eISSN 2092-7673
Effect of CMC Concentration on Cell Growth Behavior of PVA/CMC Hydrogel Ji-Yeon Shin1 Deuk Yong Lee*,1 Jung In Yoon2 Yo-Seung Song3
1
Department of Biomedical Engineering, Daelim University, Anyang 13916, Korea HANBIO Co., Ltd., Seoul 06322, Korea 3 Department of Materials Engineering, Korea Aerospace University, Goyang 10540, Korea 2
Received October 17, 2019 / Revised January 8, 2020 / Accepted January 9, 2020
Abstract: Polyvinyl alcohol (PVA)/carboxymethyl cellulose (CMC) hydrogels containing various CMC concentration of 2 to 10 wt% are prepared using cyclic freezing/thawing and subsequent γ-ray irradiation to evaluate the effect of CMC content on cell growth rate of the hydrogels. A 3-D porous network structure is successfully formed via physical and chemical crosslinking. The pore size and the metronidazole drug release rate of the PVA/CMC hydrogels increase with raising the CMC concentration from 2 to 10 wt%, however, the structural integrity is severely damaged. The highest compressive strength of 99.2 ± 9.6 kPa and the fastest cell growth determined by scratch assay and enzyme-linked immunosorbent assay are observed for the irradiated PVA/6 wt% CMC hydrogels, indicating that they are highly suitable for wound dressing requiring fast regeneration. Keywords: polyvinyl alcohol (PVA), sodium carboxymethyl cellulose (CMC), scaffold, freezing/thawing, γ-ray irradiation, cell growth, scratch assay, ELISA assay
1. Introduction Although people’s average life span and living standards have increased with the development of medical technology, traffic accidents, scar, trauma and burn patients are on the rise.1-7 Transplants of autologous or cultured fibroblasts may be the ultimate treatments of severe burns or diabetes. Due to the prolonged procedure, a dressing is needed to protect and heal the wound. Dressings for optimal wound healing do the following: absorb body fluids from the wound, prevent infection, and provide visibility via transparency.1,3,8 Polymer hydrogels, which possess interconnected pore network, high surface area, structural integrity, and appropriate biodegradation, provide desirable conditions for the treatment of skin wounds. These porous networks interconnected by physical and chemical bonds are able to include large amount of water or biological fluids as well as facilitate cell and nutrient transportation.1,3,7-10 Polyvinyl alcohol (PVA) is a semi-crystalline polymer whose hydroxyl groups produce inter- and intra-molecular hydrogen bonding. Sodium carboxymethyl cellulose (CMC) is a typical ionictype cellulose ether with multiple carboxyl groups and a polysaccharide with a double helix structure. The crosslinking may take place between PVA hydroxyl groups which are situated at the exterior part of the macromolecular coils and stretched CMC chains.2,11-13 Mechanical properties of PVA/CMC hydrogels were Acknowledgments: This work was supported by the Materials & Components Technology Development Program (Project No. 20003560), f
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